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Anti-L1-CAM Reference Antibody (Ligachem patent anti-L1-CAM)

Anti-L1-CAM Reference Antibody (Ligachem patent anti-L1-CAM) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-L1-CAM Reference Antibody (Ligachem patent anti-L1-CAM) is 97.63%, determined by SEC-HPLC.
HuL1CAM-HEK293 (A9) cell line were stained with Ligachem patent anti-L1-CAM and negative control protein respectively, washed and then followed by PE and analyzed with FACS, with the EC50 is 0.232 nM.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	P32004
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1-REM-GASD-ALIE
Calculated MW	140003 Da

Additional Information
Target/Specificity	L1-CAM
Expression system	CHO

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	L1CAM
Synonyms	CAML1, MIC5
Function	Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.
Cellular Location	Cell membrane; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:Q05695}. Cell projection, growth cone {ECO:0000250\|UniProtKB:Q05695}. Cell projection, axon. Cell projection, dendrite Note=Colocalized with SHTN1 in close apposition with actin filaments in filopodia and lamellipodia of axonalne growth cones of hippocampal neurons (By similarity). In neurons, detected predominantly in axons and cell body, weak localization to dendrites (PubMed:20621658) {ECO:0000250\|UniProtKB:Q05695, ECO:0000269\|PubMed:20621658}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol