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Anti-TEM8 Reference Antibody (Biomed Valley patent anti-TEM8)

Anti-TEM8 Reference Antibody (Biomed Valley patent anti-TEM8) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-TEM8 Reference Antibody (Biomed Valley patent anti-TEM8) is 98.85%, determined by SEC-HPLC.
RD cell line were stained with Biomed Valley patent anti-TEM8 and negative control protein respectively, washed and then followed by PE and analyzed with FACS, with the EC50 is 0.061 nM.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	Q9H6X2
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	62789 Da

Additional Information
Target/Specificity	TEM8
Expression system	CHO

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	ANTXR1 {ECO:0000303\|PubMed:22912819, ECO:0000312\|HGNC:HGNC:21014}
Function	Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton and thereby plays an important role in normal extracellular matrix (ECM) homeostasis. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells. May also act as a receptor for PLAU. Upon ligand binding, stimulates the phosphorylation of EGFR and ERK1/2 (PubMed:30241478).
Cellular Location	Cell membrane; Single-pass type I membrane protein. Cell projection, lamellipodium membrane; Single-pass type I membrane protein. Cell projection, filopodium membrane; Single-pass type I membrane protein. Note=At the membrane of lamellipodia and at the tip of actin-enriched filopodia (PubMed:16762926). Colocalizes with actin at the base of lamellipodia (PubMed:16762926)
Tissue Location	Detected in umbilical vein endothelial cells (at protein level). Highly expressed in tumor endothelial cells

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol