> 首页 > 产品 > 一抗 > 其他 > Anti-Beta Klotho Reference Antibody (Novartis patent anti-Beta Klotho)

Anti-Beta Klotho Reference Antibody (Novartis patent anti-Beta Klotho)

Anti-Beta Klotho Reference Antibody (Novartis patent anti-Beta Klotho) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-Beta Klotho Reference Antibody (Novartis patent anti-Beta Klotho) is 97.65%, determined by SEC-HPLC.
HuKLB-HEK293-A6 cell line were stained with Novartis patent anti-Beta Klotho and negative control protein respectively, washed and then followed by PE and analyzed with FACS, with the EC50 is 1.187 nM.

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	Q9UEF7
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1-LALA-REM
Calculated MW	116181 Da

Additional Information
Target/Specificity	Beta Klotho
Expression system	CHO

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	KL
Function	Exhibits beta-glucuronidase activity in vitro despite lacking canonical catalytic Glu residues of glycosyl hydrolase 1 family enzymes (By similarity). Hydrolyzes steroid beta-D-glucuronides including estradiol-3-beta-D-glucuronide, estrone-3-beta-D-glucuronide and estriol-3-beta-D-glucuronide (By similarity). Essential for the interaction between FGF23 and FGFR1 and for FGF23-mediated regulation of renal vitamin D and phosphate homeostasis (By similarity).
Cellular Location	[Isoform 1]: Cell membrane; Single-pass type I membrane protein. Apical cell membrane {ECO:0000250\|UniProtKB:O35082}; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:O35082}. Note=Isoform 1 shedding leads to a soluble peptide. {ECO:0000250\|UniProtKB:O35082} [Klotho peptide]: Secreted {ECO:0000250\|UniProtKB:O35082}
Tissue Location	Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid Expressed in kidney, placenta, small intestine and prostate. Down- regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol