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Anti-Ephrin-B2 Reference Antibody (Medimmune patent anti-Ephrin-B2)

Anti-Ephrin-B2 Reference Antibody (Medimmune patent anti-Ephrin-B2) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-Ephrin-B2 Reference Antibody (Medimmune patent anti-Ephrin-B2) is 98.74%, determined by SEC-HPLC.
PLVX-puro-Ephrin-B2-FL cell line were stained with Medimmune patent anti-Ephrin-B2 and negative control protein respectively, washed and then followed by PE and analyzed with FACS, with the EC50 is 0.2112 nM.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	P52799
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	36923 Da

Additional Information
Target/Specificity	Ephrin-B2
Expression system	CHO

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	EFNB2
Synonyms	EPLG5, HTKL, LERK5
Function	Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
Cellular Location	Cell membrane; Single-pass type I membrane protein. Cell junction, adherens junction {ECO:0000250\|UniProtKB:P52800}
Tissue Location	Lung and kidney.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol