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Anti-P2XR4 Reference Antibody (Medimmune patent anti-P2XR4)

Anti-P2XR4 Reference Antibody (Medimmune patent anti-P2XR4) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-P2XR4 Reference Antibody (Medimmune patent anti-P2XR4) is 99.46%, determined by SEC-HPLC.
PLVX-puro-P2XR4-FL cell line were stained with Medimmune patent anti-P2XR4 and negative control protein respectively, washed and then followed by PE and analyzed with FACS, with the EC50 is 10.52 nM.

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	Q99571
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	43369 Da

Additional Information
Target/Specificity	P2XR4
Expression system	CHO

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	P2RX4
Function	ATP-gated nonselective transmembrane cation channel permeable to potassium, sodium and calcium (PubMed:9016352). CTP, but not GTP or UTP, functions as a weak affinity agonist for P2RX4 (By similarity). Activated by extracellularly released ATP, it plays multiple role in immunity and central nervous system physiology (PubMed:35165166). Plays a key role in initial steps of T-cell activation and Ca(2+) microdomain formation (By similarity). Also participates in basal T-cell activity without TCR/CD3 stimulation (By similarity). Promotes the differentiation and activation of Th17 cells via expression of retinoic acid-related orphan receptor C/RORC (PubMed:35165166). Upon activation, drives microglia motility via the PI3K/Akt pathway (By similarity). Could also function as an ATP-gated cation channel of lysosomal membranes (By similarity).
Cellular Location	Cell membrane; Multi-pass membrane protein {ECO:0000250\|UniProtKB:F8W463}. Lysosome membrane; Multi-pass membrane protein

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol