> 首页 > 产品 > 一抗 > 其他 > Anti-CD86 Reference Antibody (Ludwig-Maximilians U. anti-CD86 CAR)

Anti-CD86 Reference Antibody (Ludwig-Maximilians U. anti-CD86 CAR)

Human CD317 -expressing Hela cells were stained with Anti- Human CD317,（HM1.24 ）In Vivo Mab, with isotype and secondary-only controls. Flow analysis showed specific binding of the antibody, with an EC50 of 0.545 nM.
Human CD317 -expressing Hela cells were stained with Anti- Human CD317,（HM1.24 ）In Vivo Mab. Compared to the isotype control and secondary-only control, a CD317 -positive population is apparent.
Anti- Human CD317,（HM1.24 ）In Vivo Mab on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti- Human CD317,（HM1.24 ）In Vivo Mab is 97%, determined by SEC-HPLC.

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	P42081
Reactivity	Human
Clonality	Monoclonal
Isotype	ScFv-C6His
Calculated MW	37682 Da

Additional Information
Target/Specificity	CD86
Expression system	CHO

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	CD86
Synonyms	CD28LG2
Function	Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed:12196291, PubMed:7694363). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed:12196291, PubMed:7694363). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity). Also acts as an inhibitor of T-cell activation by acting as a ligand for CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming (PubMed:11279501).
Cellular Location	Cell membrane; Single-pass type I membrane protein
Tissue Location	Expressed on the surfaces of antigen-presenting cells.