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Anti-PD-1 & CTLA-4 Reference Antibody (Cadonilimab)

Anti-PD-1 & CTLA-4 Reference Antibody (cadonilimab) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-PD-1 & CTLA-4 Reference Antibody (cadonilimab) is 98.79%, determined by SEC-HPLC.
Cadonilimab bound to PD-1 protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Cadonilimab bound to in human PD-1 Protein-His, and the EC50 was 0.09436 nM.
Cadonilimab bound to CTLA4 protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Cadonilimab bound to in human CTLA4 Protein-His, and the EC50 was 0.06733 nM.
Cadonilimab bound to huCTLA4-CHO cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Cadonilimab bound to huCTLA4-CHO cells, and the EC50 was 27.870 nM.
Cadonnilimab bound to PD-1-Jukat cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ) , and test by flow cytometry. As shown in fig, Cadonnilimab bound to PD-1-Jukat cells, and the EC50 was 1.071 nM.
The detected molecular weight of Anti-PD-1 & CTLA-4 Reference Antibody (Cadonilimab) is 198.69 kDa.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	Q15116 & P16410
Reactivity	Human
Clonality	Bispecific
Isotype	IgG-scFv

Additional Information
Target/Specificity	CTLA-4 / CD152,PDCD1 / PD-1 / CD279
Expression system	CHO

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.