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Anti-PD-L1 & VEGF Reference Antibody (Pm8002）

Anti-PD-L1 & VEGF Reference Antibody (Pm8002) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-PD-L1 & VEGF Reference Antibody (Pm8002) is 98.79%, determined by SEC-HPLC.
Pm8002 bound to VEGFA protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Pm8002 bound to in human VEGFA Protein-His, and the EC50 was 0.01613 nM.
Pm8002 bound to B7-H1 / PD-L1 / CD274 protein, and then rebounded to secondary antibodies(Anti-Human-κ+λ-HRP) , and read OD450. As shown in fig, Pm8002 bound to in human B7-H1 / PD-L1 / CD274 Protein-His, and the EC50 was 0.02648 nM.
Pm8002 bound to PD-L1-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ) , and test by flow cytometry. As shown in fig, Pm8002 bound to PD-L1-CHO-K cells, and the EC50 was 3.545 nM.
The detected molecular weight of Anti-PD-L1 & VEGF Reference Antibody (Pm8002) is 172.48 kDa.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	Q9NZQ7 & P15692
Reactivity	Human
Clonality	Bispecific
Isotype	IgG-VHH

Additional Information
Target/Specificity	B7-H1 / PD-L1 / CD274,VEGF
Expression system	CHO

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.