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Anti-CD47 & PD-1 Reference Antibody (Hx009）

Anti-CD47 & PD-1 Reference Antibody (Hx009）on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-CD47 & PD-1 Reference Antibody (Hx009）is 100%, determined by SEC-HPLC.
Hx009 bound to CD47 protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Hx009 bound to CD47-His, and the EC50 was 0.364 nM.
Hx009 bound to PD-1 protein, and then rebounded to secondary antibodies(Anti-Human-κ+λ-HRP) , and read OD450. As shown in fig, Hx009 bound to PD-1-FC, and the EC50 was 0.030 nM.
Hx009 bound to huPD-1-Jurkat cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Hx009 bound to huPD-1-Jurkat cells, and the EC50 was 0.747 nM.
The detected molecular weight of Anti-CD47 & PD-1 Reference Antibody (Hx009) is 173.16 kDa.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	Q08722 & Q15116
Reactivity	Human
Clonality	Bispecific
Isotype	IgG-Fusion Protein

Additional Information
Target/Specificity	PDCD1 / PD-1 / CD279,CD47
Expression system	CHO

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.