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Anti-CD3 & TPBG Reference Antibody (Gen1044)

Anti-CD3 & TPBG Reference Antibody (Gen1044) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-CD3 & TPBG Reference Antibody (Gen1044) is 97.30%, determined by SEC-HPLC.
Gen1044 bound to TPBG protein, and then rebounded to secondary antibodies(Anti-human-IgG-Fc-HRP) , and read OD450. As shown in fig, Gen1044 bound to huTPBG-His, and the EC50 was 0.022 nM.
Gen1044 bound to MDA-MB-231 cells, and then rebounded to fluorescent secondary antibodies(Anti-human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Gen1044 bound to MDA-MB-231 cells, and the EC50 was 1.367nM.
Gen1044 bound to huCD3e-jurkat cells, and then rebounded to fluorescent secondary antibodies(Anti-human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Gen1044 bound to huCD3e-jurkat cells, and the EC50 was 168.700 nM.
Plated and cultivated Gen1044 at 4℃ overnight, then with the addition of NF-AT-Jurkat cells for 6 hours. Bright-Lite was used to detect the fluorescent signal. As shown in fig, Gen1044 was able to activate the NF-AT signaling pathway, and the EC50 was 22.560 nM.
The detected molecular weight of Anti-CD3 & TPBG Reference Antibody (Gen1044) is 145.46 kDa.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	P07766 & Q13641
Reactivity	Human
Clonality	Bispecific
Isotype	IgG-like

Additional Information
Target/Specificity	TPBG,CD3
Expression system	CHO

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.