> 首页 > 产品 > 一抗 > 其他 > Anti-BCMA & CD3 Reference Antibody (Emb-06)

Anti-BCMA & CD3 Reference Antibody (Emb-06)

Anti-BCMA & CD3 Reference Antibody (Emb-06) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-BCMA & CD3 Reference Antibody (Emb-06) is 91.32%, determined by SEC-HPLC.
Emb-06 bound to BCMA protein, and then rebounded to secondary antibodies(Anti-human-IgG-Fc-HRP) , and read OD450. As shown in fig, Emb-06 bound to huBCMA-ECD-His, and the EC50 was 0.015 nM.
Emb-06 bound to huBCMA-HEK293 cells, and then rebounded to fluorescent secondary antibodies(Anti-human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Emb-06 bound to huBCMA-HEK293 cells, and the EC50 was 1.098 nM.
Emb-06 bound to huCD3e-jurkat cells, and then rebounded to fluorescent secondary antibodies(Anti-human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Emb-06 bound to huCD3e-jurkat cells, and the EC50 was 69.310 nM.
Co-incubation of Emb-06 with Jurkat cells, then with the addition of huBCMA-HEK293 cells for 6 hours. Bright-Lite was used to detect the fluorescent signal.As shown in fig, Emb-06 was able to activate the NF-AT signaling pathway, and the EC50 was 0.181 nM.
The detected molecular weight of Anti-CD3 & BCMA Reference Antibody (Emb-06) is 241.13 kDa.

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	Q02223 & P07766
Reactivity	Human
Clonality	Bispecific
Isotype	Fab-IgG

Additional Information
Target/Specificity	TNFRSF17 / BCMA / CD269,CD3
Expression system	CHO

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.