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Anti-4-1BB & TPBG/5t4 Reference Antibody (Apv-527)

Anti-4-1BB & TPBG/5t4 Reference Antibody (Apv-527) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-4-1BB & TPBG/5t4 Reference Antibody (Apv-527) is 98.99%, determined by SEC-HPLC.
Yh32367 bound to 4-1BB protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Yh32367 bound to hu-4-1BB-His, and the EC50 was 0.007 nM.
Yh32367 bound to Her2 protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Yh32367 bound to hu-Her2-His, and the EC50 was 0.009 nM.
Yh32367 bound to Her2 protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Yh32367 bound to hu-Her2-His, and the EC50 was 0.009 nM.
Yh32367 bound to BT474 cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Yh32367 bound to BT474 cells, and the EC50 was 1.889 nM.
Yh32367 bound to hu4-1BB-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Yh32367 bound to hu4-1BB-CHO-K cells, and the EC50 was 5.701 nM.
Co-incubation of Yh32367 with 4-1BB-NF-κB-Jurkat cells, then with the addition of BT474 cells for 6 hours. Bright-Lite was used to detect the fluorescent signal. As shown in fig, Yh32367 was able to activate the NF-κB signaling pathway, and the EC50 was 0.355 nM.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	Q07011 & Q13641
Reactivity	Human
Clonality	Bispecific
Isotype	ScFv-Fc-ScFv

Additional Information
Target/Specificity	TNFRSF9 / 4-1BB / CD137,TPBG
Expression system	CHO

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.