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Anti-LAG-3 & PD-1 Reference Antibody (Emb-02)

Anti-LAG-3 & PD-1 Reference Antibody (Emb-02) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
Anti-LAG-3 & PD-1 Reference Antibody (Emb-02) is 97.15%, determined by SEC-HPLC.
Emb-02 bound to huPD-1-Jurkat cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Emb-02 bound to huPD-1-Jurkat cells, and the EC50 was 0.645 nM.
Emb-02 bound to huLAG-3-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Emb-02 bound to huLAG-3-CHO-K cells, and the EC50 was 3.630 nM.
Emb-02 bound to LAG-3 protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Emb-02 bound human LAG-3 Protein-His, and the EC50 was 0.080 nM.
Emb-02 bound to PD-1 protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Emb-02 bound human PD-1 Protein-His, and the EC50 was 0.011 nM.
Co-incubation of Emb-02 with PD-1-NF-AT-Jurkat and CD3L-huPD-L1-CHO-K cells and incubated for 6 hours. Bright-Lite was used to detect the fluorescent signal. As shown in fig, Emb-02 was able to block the PD-1/PD-L1 signaling pathway, and the EC50 was 1.585 nM.
The detected molecular weight of Anti-LAG-3 & PD-1 Reference Antibody (Emb-02) is 237.40 kDa.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	P18627 & Q15116
Reactivity	Human
Clonality	Bispecific
Isotype	Fab-IgG

Additional Information
Target/Specificity	PDCD1 / PD-1 / CD279,LAG3 / CD223
Expression system	CHO

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.