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Anti-CD3 & DLL3 Reference Antibody (Tarlatamab)

Anti-CD3 & DLL3 Reference Antibody (Tarlatamab) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-CD3 & DLL3 Reference Antibody (Tarlatamab) is 94.53%, determined by SEC-HPLC.
Tarlatamab bound to DLL3 protein, and then rebounded to secondary antibodies(Anti-human-IgG-Fc-HRP) , and read OD450. As shown in fig, Tarlatamab bound to huPD-1-his, and the EC50 was 0.010 nM
Tarlatamab bound to huCD3e-jurkat cells, and then rebounded to fluorescent secondary antibodies(Anti-human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Tarlatamab bound to huCD3e-jurkat cells, and the EC50 was 6.141 nM.
Tarlatamab bound to hu-DLL3-HEK293 cells, and then rebounded to fluorescent secondary antibodies(Anti-human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Tarlatamab bound to hu-DLL3-HEK293 cells, and the EC50 was 0.337 nM.
Co-incubation of Tarlatamab with Jurkat cells, then with the addition of hu-DLL3-HEK293 cells for 6 hours. Bright-Lite was used to detect the fluorescent signal.As shown in fig, Tarlatamab was able to activate the NF-AT signaling pathway, and the EC50 was 0.056 nM.
The detected molecular weight of Anti-CD3 & DLL3 Reference Antibody (Tarlatamab) is 91.84 kDa. The antibody sequence matches the sequence in patent (WO2017021349).The Mass data suggested a smaller molecular weight of Tarlatamab (91.84 kDa) than the theoretical value (105.2 kDa, from the above patent). Functional assays domenstrated Tarlatamab retains the function of CD3 and DLL3.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	P07766 & Q9NYJ7
Reactivity	Human
Clonality	Bispecific
Isotype	HLE-BiTE

Additional Information
Target/Specificity	DLL3,CD3
Expression system	CHO

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.