癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Nop30 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | O60936 |
| Other Accession | AAH12798, 15215394 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 22629 Da |
| Conjugate | Unconjugated |
| Application Notes | Nop30 antibody can be used for detection of Nop30 by Western blot at 0.5 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 0.5 µg/mL. |
| Gene ID | 8996 |
|---|---|
| Other Names | Nop30 Antibody: ARC, FCM, MYP, NOP, NOP30, ARC, Nucleolar protein 3, Apoptosis repressor with CARD, Myp, nucleolar protein 3 (apoptosis repressor with CARD domain) |
| Target/Specificity | NOL3; |
| Reconstitution & Storage | Nop30 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | Nop30 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | NOL3 (HGNC:7869) |
|---|---|
| Function | [Isoform 1]: May be involved in RNA splicing. |
| Cellular Location | [Isoform 1]: Nucleus, nucleolus. Note=The SR-rich C-terminus mediates nuclear localization. [Isoform 2]: Cytoplasm. Mitochondrion {ECO:0000250|UniProtKB:Q62881}. Sarcoplasmic reticulum {ECO:0000250|UniProtKB:Q62881}. Membrane; Lipid-anchor. Note=Phosphorylation at Thr-149 results in translocation to mitochondria. Colocalized with mitochondria in response to oxidative stress. {ECO:0000250|UniProtKB:Q62881} |
| Tissue Location | Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Nop30 Antibody: Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and the caspase family of proteases. CARD domain containing cell death regulators include RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD. An alternate splicing isoform of ARC was identified as Nop30. While ARC interacts with caspase-2 and -8 and suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1 and DR3, Nop30 multimerizes and binds to the splicing factor SRp30c and may act to influence alternative splice site selection in vivo. The Nop30 antibody will not detect ARC protein.
REFERENCES
Jin Z and El Deiry WS. Overview of cell death signaling pathways. Cancer Biol. Ther. 2004; 4:139-63.
Koseki T, Inohara N, Chen S, et al. ARC, an inhibitor of apoptosis expressed in skeletal muscle and heart that interacts selectively with caspases. Proc. Natl. Acad. Sci. USA 1998; 95:5156-60.
Stoss O, Schwaiger FW, Cooper TA, et al. Alternative splicing determines the intracellular localization of the novel nuclear protein Nop30 and its interaction with the splicing factor SRp30c. J. Biol. Chem.1999; 274:10951-62
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