癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
PARC Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, ICC, E |
|---|---|
| Primary Accession | Q8IWT3 |
| Other Accession | NP_055904, 24307991 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 281229 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | PARC antibody can be used for the detection of PARC by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 1 µg/mL. For immunofluorescence start at 2 µg/mL. |
| Gene ID | 23113 |
|---|---|
| Other Names | PARC Antibody: PARC, H7AP1, KIAA0708, PARC, Cullin-9, UbcH7-associated protein 1, CUL-9, cullin 9 |
| Target/Specificity | CUL9; |
| Reconstitution & Storage | PARC antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | PARC Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | CUL9 |
|---|---|
| Synonyms | H7AP1, KIAA0708, PARC |
| Function | Core component of a Cul9-RING ubiquitin-protein ligase complex composed of CUL9 and RBX1 (PubMed:38605244). The CUL9-RBX1 complex mediates ubiquitination and subsequent degradation of BIRC5 and is required to maintain microtubule dynamics and genome integrity. Acts downstream of the 3M complex, which inhibits the ubiquitination of BIRC5 (PubMed:24793696). The CUL9-RBX1 complex also mediates mono- ubiquitination of p53/TP53 (PubMed:38605244). Acts as a cytoplasmic anchor protein in p53/TP53-associated protein complex. Regulates the subcellular localization of p53/TP53 and its subsequent function (PubMed:12526791, PubMed:17332328). Ubiquitinates apurinic/apyrimidinic endodeoxyribonuclease APEX2 (PubMed:38605244). Ubiquitination by the CUL9-RBX1 complex is predominantly mediated by E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2D2 (PubMed:38605244). |
| Cellular Location | Cytoplasm. |
| Tissue Location | Ubiquitously expressed in all tissues with highest expression in testis brain and kidney. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
PARC Antibody: The continued localization of p53 to the nucleus is essential for its function as a tumor suppressor. PARC, a large, Parkin-like ubiquitin ligase has recently been identified as a cytoplasmic anchor protein in p53-associated protein complexes. In the absence of stress, PARC inactivation results in nuclear localization of p53 and activation of p53-dependent apoptosis, while overexpression of this protein promoted cytoplasmic sequestration of p53. Surprisingly, PARC knockout mice were viable and exhibited no obvious phenotype, suggesting that other proteins, such as the highly related cullin family of E3 ubiquitin ligases, may perform similar functions in the absence of PARC. Additionally, it has been suggested that p53 binding to PARC may serve to control PARC function.
REFERENCES
Nikolaev AY, Li M, Puskas N, et al. Parc: a cytoplasmic anchor for p53. Cell 2003; 112:29-40.
Skaar JR, Arai T, and DeCaprio JA. Dimerization of CUL7 and PARC is not required for all CUL7 functions and mouse development. Mol. Cell. Biol. 2005; 25:5579-89.
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