癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
TWEAK Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E, IHC-P |
|---|---|
| Primary Accession | O43508 |
| Other Accession | NP_003800, 4507597 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 27216 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | TWEAK antibody can be used for the detection of TWEAK by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 10 µg/mL. |
| Gene ID | 8742 |
|---|---|
| Other Names | TWEAK Antibody: APO3L, DR3LG, TWEAK, APO3L, UNQ181/PRO207, Tumor necrosis factor ligand superfamily member 12, APO3 ligand, tumor necrosis factor (ligand) superfamily, member 12 |
| Target/Specificity | TNFSF12; |
| Reconstitution & Storage | TWEAK antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | TWEAK Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | TNFSF12 |
|---|---|
| Synonyms | APO3L, DR3LG |
| Function | Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion. |
| Cellular Location | Cell membrane; Single-pass type II membrane protein [Isoform TWE-PRIL]: Cell membrane; Single-pass membrane protein |
| Tissue Location | Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
TWEAK Antibody: TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains.
REFERENCES
Wiley SR and Winkles JA. TWEAK, a member of the TNF superfamily, is a multifunctional cytokine that binds the TweakR/Fn14 receptor. Cyto. And Growth Factor Rev. 2003; 14:241-9.
Chicheportiche Y, Bourdon PR, Xu H, et al. TWEAK, a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. J. Biol. Chem. 1997; 272:32401-10.
Lynch CN, Wang YC, Lund JK, et al. TWEAK induces angiogenesis and proliferation of endothelial cells. J. Biol. Chem. 1999; 274:8455-9.
Chicheportiche Y, Chicheportiche R, Sizing I, et al. Proinflammatory activity of TWEAK on human dermal fibroblasts and synoviocytes: blocking and enhancing effects of anti-TWEAK monoclonal antibodies. Arthr. Res. 2002; 4:126-33. 5.
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