癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
SAPAP1 Antibody
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Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | O60500 |
| Other Accession | NP_004737, 4758822 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 134742 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | SAPAP1 antibody can be used for detection of SAPAP1 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 4868 |
|---|---|
| Other Names | Nephrin, Renal glomerulus-specific cell adhesion receptor, NPHS1, NPHN |
| Target/Specificity | NPHS1; At least three isoforms of SAPAP1 are known to exist. This SAPAP1 antibody will not cross-react with other SAPAP proteins. |
| Reconstitution & Storage | SAPAP1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | SAPAP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NPHS1 |
|---|---|
| Synonyms | NPHN |
| Function | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity). |
| Cellular Location | Cell membrane; Single-pass type I membrane protein. Note=Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane. |
| Tissue Location | Specifically expressed in podocytes of kidney glomeruli |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
SAPAP1 Antibody: SAP90/PSD-95-associated protein 1 (SAPAP1, also known as DLGAP1 or GKAP) is a member of a protein family whose members specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. Like the other SAPAP proteins, SAPAP1 is thought to be an adaptor protein that also interacts with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). SAPAP1 mRNA is targeted to cell bodies in a similar manner to SAPAP2 and -4, whereas SAPAP3 mRNA is detected mainly in cell bodies. SAPAP1 protein however, is targeted to the synapse and is not reliant on the synaptic localization of PSD-95 or the synaptic scaffolding molecule (S-SCAM).
REFERENCES
SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density. J. Biol. Chem.1997; 272:11943-51.
Kindler S, Rehbein M, Classen B, et al. Distinct spatiotemporal expression of SAPAP transcripts in the developing rat brain: a novel dendritically localized mRNA. Brain Res. Mol. Brain Res.2004; 126:14-21.
Bongiorno-Borbone L, Kadare G, Benfenati F, et al. FAK and PYK2 interact with SAP/PSD-95-associated protein-3. Biochem. Biophys. Res. Commun.2005; 337:641-6.
Welch JM, Wang D, and Feng G. Differential mRNA expression and protein localization of the SAP90/PSD-95-associated proteins (SAPAPs) in the nervous system of the mouse. J. Comp. Neurol.2004; 472:24-39.
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