癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
MED4 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E, IHC-P |
|---|---|
| Primary Accession | Q9NPJ6 |
| Other Accession | NP_054885, 7661788 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 29745 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | MED4 antibody can be used for detection of MED4 by Western blot at 0.5 µg/mL. Antibody can also be used for immunohistochemistry starting at 10 µg/mL. |
| Gene ID | 29079 |
|---|---|
| Other Names | Mediator of RNA polymerase II transcription subunit 4, Activator-recruited cofactor 36 kDa component, ARC36, Mediator complex subunit 4, TRAP/SMCC/PC2 subunit p36 subunit, Vitamin D3 receptor-interacting protein complex 36 kDa component, DRIP36, MED4, ARC36, DRIP36, VDRIP |
| Target/Specificity | MED4; At least three isoforms of MED4 are known to exist; this antibody only recognizes the largest isoform. |
| Reconstitution & Storage | MED4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | MED4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | MED4 |
|---|---|
| Synonyms | ARC36, DRIP36, VDRIP |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. |
| Cellular Location | Nucleus. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
MED4 Antibody: The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED4. This protein has also been shown to be part of the vitamin D receptor interacting complex. MED4 was recently identified as an HIV dependency factor (HDF), suggesting that MED4 may be an important drug target in HIV treatment.
REFERENCES
Sato S, Tomomori-Sato C, Parmely TJ, et al. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Mol. Cell2004; 14:685-91.
Gustafsson CM, Myers LC, Beve J, et al. Identification of new mediator subunits in the RNA polymerase II holoenzyme from Saccharomyces cerevisiae. J. Biol. Chem.1998; 273:30851-4.
Rachez C, Lemon BD, Suldan Z, et al. Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. Nature1999; 398:824-8.
Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.
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