癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
TRIM30 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | P15533 |
| Other Accession | NP_033125, 125347379 |
| Reactivity | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 57330 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | TRIM30 antibody can be used for detection of TRIM30 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 20128 |
|---|---|
| Other Names | Tripartite motif-containing protein 30A, Down regulatory protein of interleukin-2 receptor, Tripartite motif-containing protein 30, Trim30a, Rpt-1, Rpt1, Trim30 |
| Target/Specificity | Trim30; |
| Reconstitution & Storage | TRIM30 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | TRIM30 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Trim30a |
|---|---|
| Synonyms | Rpt-1, Rpt1, Trim30 |
| Function | E3 ubiquitin-protein ligase that acts as a negative regulator of innate immune responses, and which is required to prevent excessive inflammation (PubMed:18345001, PubMed:19147168, PubMed:21048113, PubMed:26114947). Negatively regulates Toll-like receptor (TLR)- mediated activation of NF-kappa-B by promoting ubiquitination and degradation of TAB2 and TAB3 and preventing TRAF6 autoubiquitination (PubMed:18345001). Negatively regulates production of reactive oxygen species (ROS) which inhibits activation of the NLRP3 inflammasome complex (PubMed:21048113). This, in turn, regulates activation of CASP1 and subsequent cleavage of IL1B and IL18 (PubMed:21048113). Acts as a negative regulator of cGAS-STING signaling by mediating ubiquitination and degradation of STING1 (PubMed:26114947). |
| Cellular Location | Cytoplasm. Nucleus. |
| Tissue Location | Highly expressed in spleen and lymph nodes (at protein level). |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
TRIM30 Antibody: TRIM30 belongs to a family of the tripartite motif (TRIM) proteins involved in the regulation of cell proliferation, differentiation, development, oncogenesis, apoptosis and antiviral responses. The TRIM protein family is an expanding family of RING ('really interesting new gene') proteins, also known as RBCC proteins as they contain an RBCC motif, which comprises a RING domain, one or two B-boxes and a predicted coiled-coil region. Studies have shown that some TRIM family members are critical to innate immunity; TRIM5, TRIM19 and TRIM25, for example, have been shown to restrict viral infection. A recent study shows that TRIM30 functions as a negative modulator of the TLR signaling pathway, by targeting TAB2 and TAB3, and contributes to the inhibition of TLR-mediated NF-κB activation. The importance of TRIM30 in the attenuation or termination of NF-κB activation suggests that targeting of TAB2 and TAB3 by TRIM30α may be a mechanism for modulating many types of immune responses.
REFERENCES
Nisole S, Stoye JP, and Saib A. TRIM family proteins: retroviral restriction and antiviral defence. Nat. Rev. Microbiol.2005; 3:799-808.
Sakuma R, Noser JA, Ohmine S, et al. Rhesus monkey TRIM5 alpha restricts HIV-1 production through rapid degradation of viral Gag polyproteins. Nat. Med.2007; 13:631-635.
Gack MU, Shin YC, Joo CH, et al. TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity. Nature2007; 446:916-920.
Patarca R, Freeman GJ, Schwartz J, et al. rpt-1, an intracellular protein from helper/inducer T cells that regulates gene expression of interleukin 2 receptor and human immunodeficiency virus type 1. Proc. Natl. Acad. Sci. USA1988; 85:2733-7.
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