癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
ZBRK1 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | Q9GZX5 |
| Other Accession | NP_067645, 59710107 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 60011 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | ZBRK1 antibody can be used for detection of ZBRK1 by Western blot at 1 µg/mL. |
| Gene ID | 59348 |
|---|---|
| Other Names | Zinc finger protein 350, KRAB zinc finger protein ZFQR, Zinc finger and BRCA1-interacting protein with a KRAB domain 1, Zinc finger protein ZBRK1, ZNF350, ZBRK1 |
| Target/Specificity | ZNF350; |
| Reconstitution & Storage | ZBRK1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | ZBRK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | ZNF350 |
|---|---|
| Synonyms | ZBRK1 |
| Function | Transcriptional repressor. Binds to a specific sequence, 5'- GGGxxxCAGxxxTTT-3', within GADD45 intron 3. |
| Cellular Location | Nucleus. Nucleus matrix. Note=Associated with the nuclear matrix |
| Tissue Location | Widely expressed.. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
ZBRK1 Antibody: ZBRK1 is a member of the Kruppel-associated box-zinc finger protein (KRAB-ZFP) protein family of transcriptional repressors. ZBRK1 has been shown to not only be up-regulated in senescent fibroblasts but also interacts with BRCA1, a transcriptional regulator with tumor suppressor activity. ZBRK1 binds to a specific sequence within the GADD45 gene, supporting the assembly of a nuclear complex containing BRCA1 and repressing its transcription. Recent experiments demonstrate that ZBRK1 can homo-oligomerize in vitro and in vivo through a C-terminal transcriptional repression domain (CTRD), and this oligomerization facilitates the ZBRK1-directed transcriptional repression through ZBRK1 response elements. These results suggest that higher order ZBRK1 oligomers may assemble through both protein-DNA and CTRD-dependent protein-protein interactions.
REFERENCES
Zheng L, Pan H, Li S, et al. Sequence-specific transcription corepressor function for BRCA1 through a novel Zinc finger protein, ZBRK1. Mol. Cell2000; 6:757-68.
Collins T, Stone JR, and Williams AJ. All in the family: the BTB/POZ, KRAB, and SCAN domains. Mol. Cell. Biol.2001; 21:3609-15.
Tan W, Kim S, and Boyer TG. Tetrameric oligomerization mediates transcriptional repression by the BRCA1-dependent Kruppel-associated Box-Zinc finger protein ZBRK1. J. Biol. Chem.2004; 279:55153-60.
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