癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
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Spred3 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | Q2MJR0 |
| Other Accession | NP_001035987, 110225353 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 42670 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | Spred3 antibody can be used for detection of Spred3 by Western blot at 2 - 4 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 399473 |
|---|---|
| Other Names | Sprouty-related, EVH1 domain-containing protein 3, Spred-3, SPRED3, EVE-3 |
| Target/Specificity | SPRED3; This Spred3 antibody is predicted to have no cross-reactivity to Spred1 or Spred2. |
| Reconstitution & Storage | Spred3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | Spred3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SPRED3 |
|---|---|
| Synonyms | EVE-3 |
| Function | Tyrosine kinase substrate that inhibits growth-factor- mediated activation of MAP kinase (By similarity). Inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation, probably by inhibiting FGF-mediated phosphorylation of ERK1/2 (By similarity). Inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells (By similarity). |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:Q6P6N5}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q6P6N5} |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Spred3 Antibody: Spred3 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1 and Spred2, Spred3 contains and EVH1 domain in its amino terminus and a Sprouty-related cysteine-rich (SPR) domain in its C-terminus, but does not possess a functional c-kit binding domain (KBD). The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Although Spred3 was initially reported to be expressed in brain, at least one report also indicates that Spred3 can be found in liver. At least two isoforms of Spred3 are known to exist.
REFERENCES
Kato R, Nonami A, Taketomi T, et al. Molecular cloning of mammalian Spred-3 which suppresses tyrosine kinase-mediated Erk activation. Biochem. Biophys. Res. Commun.2003; 302:767-72.
Casci T, Vinos J, and Freeman M. Sprouty, an intracellular inhibitor of Ras signaling. Cell1999; 96:655-65.
Minowada G, Jarvis LA, Chi CL, et al. Vertebrate Sprouty genes are induced by FGF signaling and can cause chondrodysplasia when overexpressed. Development1999; 126:4465-75.
Lee SH, Schloss DJ, Jarvis L, et al. Inhibition of angiogenesis by a mouse sprouty protein. J. Biol. Chem.2001; 276:4128-33.
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