癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Gle1 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | Q53GS7 |
| Other Accession | NP_001003722, 51317384 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 79836 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | Gle1 antibody can be used for detection of Gle1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 2733 |
|---|---|
| Other Names | Nucleoporin GLE1, hGLE1, GLE1-like protein, GLE1, GLE1L |
| Target/Specificity | GLE1; |
| Reconstitution & Storage | Gle1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | Gle1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | GLE1 |
|---|---|
| Synonyms | GLE1L |
| Function | Required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm. May be involved in the terminal step of the mRNA transport through the nuclear pore complex (NPC). |
| Cellular Location | Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm (PubMed:12668658). Shuttling is essential for its mRNA export function (PubMed:12668658). |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Gle1 Antibody: The proper expression of gene products in eukaryotic cells relies on efficient transport of mRNA molecules out of the nucleus. Gle1 is an essential mRNA export factor in both human and yeast cells. It associates with the nuclear pore complex (NPC) through hCG1 and NUP155 in mammalian cells and in conjunction with inositol hexakisphosphate (IP6), stimulates Dbp5, a member of the DEAD-box helicase family, triggering mRNP remodeling and facilitating RNA export from the nucleus. Recent evidence suggests that mutations in Gle1 causing defects in mRNA export can result in human disease. At least three isoforms of Gle1 are known to exist.
REFERENCES
Murphy R and Wente SR. An RNA-export mediator with an essential nuclear export signal. Nature1996; 383:357-60.
Watkins JL, Murphy R, Emtage JL, et al. The human homologue of Saccharomyces cerevisiae Gle1p is required for poly(A)+ RNA export. Proc. Natl. Acad. Sci. USA1998; 95:6779-84.
Kendirgi F, Rexer DJ, Alcazar-Roman AR, et al. Interaction between the shuttling mRNA export factor Gle1 and the nucleoporin hCG1: A conserved mechanism in the export of Hsp70 mRNA. Mol. Biol. Cell2005; 16:4304-15.
Rayala HJ, Kendirgi F, Barry DM, et al. The mRNA export factor human Gle1 interacts with the nuclear pore complex protein Nup155. Mol. Cell. Proteomics2004; 3:145-55.
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