癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
SH3BP4 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E, IHC-P |
|---|---|
| Primary Accession | Q9P0V3 |
| Other Accession | NP_055336, 7657562 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 107496 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | SH3BP4 antibody can be used for detection of SH3BP4 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. |
| Gene ID | 23677 |
|---|---|
| Other Names | SH3 domain-binding protein 4, EH-binding protein 10, Transferrin receptor-trafficking protein, SH3BP4, BOG25, EHB10, TTP |
| Target/Specificity | SH3BP4; |
| Reconstitution & Storage | SH3BP4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | SH3BP4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SH3BP4 |
|---|---|
| Synonyms | BOG25, EHB10, TTP |
| Function | May function in transferrin receptor internalization at the plasma membrane through a cargo-specific control of clathrin-mediated endocytosis. Alternatively, may act as a negative regulator of the amino acid-induced TOR signaling by inhibiting the formation of active Rag GTPase complexes. Preferentially binds inactive Rag GTPase complexes and prevents their interaction with the mTORC1 complex inhibiting its relocalization to lysosomes and its activation. Thereby, may indirectly regulate cell growth, proliferation and autophagy. |
| Cellular Location | Membrane, clathrin-coated pit. Cytoplasmic vesicle, clathrin-coated vesicle. Nucleus Note=Specifically associated with transferrin receptor-containing clathrin-coated pits and clathrin-coated vesicles. May also localize to the nucleus |
| Tissue Location | Expressed in all tissues tested with higher expression in pancreas. Expressed by retinal pigment epithelial cells (at protein level). |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
SH3BP4 Antibody: SH3BP4 was initially isolated as a novel cDNA using differential display analysis using cultured human corneal fibroblasts. The protein contains three Asn-Pro-Phe (NPF) motifs, an SH3 domain, a PXXP motif, a bipartite nuclear targeting signal, and a tyrosine phosphorylation site. SH3BP4 has been shown to interact with specific endocytic proteins such as clathrin, dynamin, and the transferrin receptor (TfR) and localizes to TfR-containing coated pits and vesicles. It is thought to be involved in cargo-specific control of clathrin-mediated endocytosis, specifically controlling the internalization of TfR. At least two isoforms of SH3BP4 are known to exist.
REFERENCES
Dunlevy JR, Berryhill BL, Vergnes JP, et al. Cloning, chromosomal localization, and characterization of cDNA from a novel gene, SH3BP4, expressed by human corneal fibroblasts. Genomics1999; 62:519-24.
Tosoni D, Puri C, Confalonieri S, et al. TTP specifically regulates the internalization of the transferrin receptor. Cell2005; 123:875-888.
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