癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
IRGC Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, ICC, E |
|---|---|
| Primary Accession | Q6NXR0 |
| Other Accession | NP_062558, 10257429 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 50288 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | IRGC antibody can be used for detection of IRGC by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 56269 |
|---|---|
| Other Names | Interferon-inducible GTPase 5, 3.6.5.-, Immunity-related GTPase cinema 1, IRGC, IIGP5, IRGC1 |
| Target/Specificity | IRGC; Two isoforms of IRGC are known to exist; this antibody will recognize both isoforms. |
| Reconstitution & Storage | IRGC antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | IRGC Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | IRGC (HGNC:28835) |
|---|---|
| Synonyms | IIGP5, IRGC1 |
| Function | GTPase required for sperm motility and therefore male fertility, via positive regulation of spermatozoa fibrous sheath formation. |
| Cellular Location | Cell projection, cilium, flagellum. Lipid droplet. Note=Expressed in the sperm mitochondrial sheath. |
| Tissue Location | Abundantly expressed in semen (at protein level). |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
IRGC Antibody: Immunity-related GTPases (IRG) (also known as p47 GTPases) are a family of GTPase proteins found in vertebrates, which play critical roles in mediating innate resistance to intracellular pathogens. IRG genes have been found in a number of mammals and lower species including mice, rats, zebrafish and humans. Most of the mouse genes contain interferon-stimulated response elements which mediate transcriptional activation by IFNs. In humans, only two IRG genes have been found: human IRGC encodes a full-length IRG protein that, like the mouse homologue, is constitutively expressed in testis, while human IRGM encodes a considerably truncated protein that is constitutively expressed in cultured cells including some macrophage cell lines. As the two human genes IRGC and IRGM are not subject to IFN control, it has been suggested that the host resistance mechanism supported by IRG proteins in the mouse is lacking in humans.
REFERENCES
Taylor GA. IRG proteins: key mediators of interferon-regulated host resistance to intracellular pathogens. Cell Microbiol.2007; 9:1099-107.
Yamada K, Akimoto H, Ogawa Y, et al. Upregulation of immunity-related GTPase (IRG) proteins by TNF-alpha in murine astrocytes. Biochem. Biophys. Res. Commun.2009; 382:434-9.
Singh SB, et al. Human IRGM induces autophagy to eliminate intracellular mycobacteria. Science2006; 313:1438-41.
Bekpen C, Hunn JP, Rohde C, et al. The interferon-inducible p47 (IRG) GTPases in vertebrates: loss of the cell autonomous resistance mechanism in the human lineage. Genome Biol.2005; 6:R92.
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