癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
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RUSC1 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | Q9BVN2 |
| Other Accession | NP_001098673, 157412245 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 96444 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | RUSC1 antibody can be used for detection of RUSC1 by Western blot at 1 µg/mL. |
| Gene ID | 23623 |
|---|---|
| Other Names | RUN and SH3 domain-containing protein 1, New molecule containing SH3 at the carboxy-terminus, Nesca, RUSC1, NESCA |
| Target/Specificity | RUSC1; |
| Reconstitution & Storage | RUSC1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | RUSC1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | RUSC1 {ECO:0000303|PubMed:30262884, ECO:0000312|HGNC:HGNC:17153} |
|---|---|
| Function | Associates with the adapter-like complex 4 (AP-4) and may therefore play a role in vesicular trafficking of proteins at the trans-Golgi network (PubMed:30262884). Signaling adapter which plays a role in neuronal differentiation (PubMed:15024033). Involved in regulation of NGF-dependent neurite outgrowth (PubMed:15024033). May play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins (By similarity). Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK (PubMed:15024033). Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway (PubMed:19365808). |
| Cellular Location | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q8BG26}. Cytoplasmic vesicle {ECO:0000250|UniProtKB:Q8BG26}. Early endosome {ECO:0000250|UniProtKB:Q8BG26}. Postsynaptic density {ECO:0000250|UniProtKB:Q8BG26}. Golgi apparatus {ECO:0000250|UniProtKB:Q8BG26}. Note=Translocated to the nuclear envelope upon stimulation with NGF (PubMed:15024033). Associated with membranes and microtubules (By similarity) {ECO:0000250|UniProtKB:Q8BG26, ECO:0000269|PubMed:15024033} |
| Tissue Location | Predominantly expressed in brain. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
RUSC1 Antibody: RUSC1, also known as NESCA, shares with the related protein RUSC2 a common domain structure of RUN, leucine zipper and SH3 domain in addition to over 30% amino acid identity. RUSC1 is an adapter protein that can bind to the TrkA receptor and is necessary in the NGF-induced neurite growth of PC12 cells. RUSC1 has also been shown to interact with IκB kinase- (IKK-) gamma, the regulatory subunit of the IKK complex that is required for NF-κB activation in many signaling pathways such as TNF-R or the TLR pathways. RUSC1 can also bind to the E3 ubiquitin ligase TRAF6, which then catalyzes RUSC1 polyubiquitination. Since overexpression of RUSC1 strongly inhibits TRAF6-mediated polyubiquitination of IKK-gamma, RUSC1 may be a link in the IKK-gamma-mediated NF-κB activation pathway.
REFERENCES
Matsuda S, Miyazaki K, Ichigotani Y, et al. Molecular cloning and characterization of a novel human gene (NESCA) which encodes a putative adapter protein containing SH3. Biochim. Biophys. Acta2000; 1491:321-6.
Katoh M and Katoh M. Characterization of RUSC1 and RUSC2 genes in silico. Oncol. Rep.2004; 12:933-8.
MacDonald JI, Kubu CJ, and Meakin SO. Nesca, a novel adapter, translocates to the nuclear envelope and regulates neurotrophin-induced neurite outgrowth. J. Cell Biol.2004; 164:851-62.
Napolitano G, Mirra S, Monfregola J, et al. NESCA: a new NEMO/IKKgamma and TRAF6 interacting protein. J. Cell. Phys.2009; 220:410-7.
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