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ENC-2 Antibody
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- 实验流程
- 背景知识
Application 
| WB, IF, ICC, E |
|---|---|
| Primary Accession | Q9H0H3 |
| Other Accession | AAV51405, 55418570 |
| Reactivity | Human |
| Host | Chicken |
| Clonality | Polyclonal |
| Isotype | IgY |
| Calculated MW | 65923 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | ENC-2 antibody can be used for detection of ENC-2 by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 64410 |
|---|---|
| Other Names | Kelch-like protein 25, Ectoderm-neural cortex protein 2, ENC-2, KLHL25, ENC2 |
| Target/Specificity | KLHL25; |
| Reconstitution & Storage | ENC-2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | ENC-2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | KLHL25 {ECO:0000303|PubMed:22578813, ECO:0000312|HGNC:HGNC:25732} |
|---|---|
| Function | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex involved in various processes, such as translation homeostasis and lipid synthesis (PubMed:22578813, PubMed:27664236, PubMed:34491895). The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low (PubMed:22578813). The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E (PubMed:22578813). The BCR(KLHL25) complex also acts as a regulator of lipid synthesis by mediating ubiquitination and degradation of ACLY, thereby inhibiting lipid synthesis (PubMed:27664236, PubMed:34491895). BCR(KLHL25)-mediated degradation of ACLY promotes fatty acid oxidation and is required for differentiation of inducible regulatory T (iTreg) cells (PubMed:34491895). |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
ENC-2 Antibody: The ectoderm-neural cortex-2 (ENC-2) protein a member of the BTB/PDZ Kelch protein family displaying significant homology to ENC-1, an actin-binding protein involved in neural fate specification during embryogenesis and adipocyte maturation. The exact function of ENC-2 has yet to be determined, but it has been suggested to be involved in the differentiation of neuronal precursor cells into neurons and astrocytes.
REFERENCES
Hernandez MC, Andres-Barquin PJ, Martinez S, et al. ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein. J. Neurosci.1997; 17:3038-51.
Zhao L, Gregoire F and Sul HS. Transient induction of ENC-1, a Kelch-related actin-binding protein, is required for adipocyte differentiation. J. Biol. Chem.2000; 275:16845-50.
Ahn JI, Kim SY, Ko MJ, et al. Analysis of gene expression in mouse spinal cord-derived neural precursor cells during neuronal differentiation. Gen. and Infor.2009; 7:85-96.
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