癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
SIRT5 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | Q9NXA8 |
| Other Accession | NP_036373, 6912664 |
| Reactivity | Human, Mouse |
| Host | Chicken |
| Clonality | Polyclonal |
| Isotype | IgY |
| Calculated MW | 33881 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | SIRT5 antibody can be used for detection of SIRT5 by Western blot at 1 - 2 µg/mL. |
| Gene ID | 23408 |
|---|---|
| Other Names | NAD-dependent protein deacylase sirtuin-5, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03160}, 3.5.1.- {ECO:0000255|HAMAP-Rule:MF_03160}, Regulatory protein SIR2 homolog 5 {ECO:0000255|HAMAP-Rule:MF_03160}, SIR2-like protein 5 {ECO:0000255|HAMAP-Rule:MF_03160}, SIRT5 {ECO:0000255|HAMAP-Rule:MF_03160}, SIR2L5 |
| Target/Specificity | SIRT5; |
| Reconstitution & Storage | SIRT5 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | SIRT5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SIRT5 {ECO:0000255|HAMAP-Rule:MF_03160} |
|---|---|
| Synonyms | SIR2L5 |
| Function | NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:21908771, PubMed:22076378, PubMed:24703693, PubMed:29180469). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:22076378, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed:24140062). Activates SHMT2 by mediating its desuccinylation (PubMed:29180469). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX. |
| Cellular Location | Mitochondrion matrix. Mitochondrion intermembrane space. Cytoplasm, cytosol. Nucleus. Note=Mainly mitochondrial. Also present extramitochondrially, with a fraction present in the cytosol and very small amounts also detected in the nucleus [Isoform 2]: Mitochondrion {ECO:0000255|HAMAP- Rule:MF_03160, ECO:0000269|PubMed:21143562} |
| Tissue Location | Widely expressed.. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
SIRT5 Antibody: The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and have important functions in the regulation of metabolism, growth and differentiation, inflammation, cellular survival, as well as in senescence and lifespan extension. Sirtuins, including SIRT1-7, are human homologs of yeast Sir2p. Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) which regulate cellular metabolism, e.g. energy metabolism, and thereby are associated with aging and several age-related diseases. SIRT5 localizes to mitochondria, deacetylates carbamoyl phosphate synthetase 1, and is involved in the regulation of the urea cycle.
REFERENCES
Salminen A. SIRT1: regulation of longevity via autophagy. Cell Signal2009; 21:1356-60.
Afshar G and Murnane JP. Characterization of a human gene with sequence homology to Saccharomyces cerevisiae Sir 2. Gene1999; 234:161-8.
Guarente L. Sirtuins as potential targets for metabolic syndrome. Nature2006; 444:868-74.
Vaziri H, Dessain SK, Ng Eaton E, et al. hSIR2 (SIRT1) functions as an NAD-dependent p53 deacetylase. Cell2001; 107:149-59.
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