癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
SCUBE2 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, ICC, E |
|---|---|
| Primary Accession | Q9NQ36 |
| Other Accession | NP_066025, 283046663 |
| Reactivity | Human, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 109957 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | SCUBE2 antibody can be used for detection of SCUBE2 by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 20 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 57758 |
|---|---|
| Other Names | Signal peptide, CUB and EGF-like domain-containing protein 2, Protein CEGP1, SCUBE2, CEGP1 |
| Target/Specificity | SCUBE2; |
| Reconstitution & Storage | SCUBE2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | SCUBE2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | SCUBE2 {ECO:0000303|PubMed:12270931, ECO:0000312|HGNC:HGNC:30425} |
|---|---|
| Function | Lipid-binding protein carrier required for sonic hedgehog (SHH) long-range signaling: binds to the dually lipid-modified Sonic hedgehog protein N-product (ShhN) and promotes its release from producing cells, allowing it to act as a morphogen for distant signaling (PubMed:19480626, PubMed:22677548, PubMed:22902404, PubMed:35231446). ShhN is then transferred to the CDON-BOC heterodimer and GAS1 coreceptors to reach its target, the patched receptor (PTCH1 or PTCH2), followed by activation of the smoothened signaling pathway (PubMed:35231446). May also enhance the proteolytic processing (shedding) of the lipid-modified N- and C- terminal of ShhNp at the cell surface (PubMed:24522195). Synergizes with DISP1 to increase SHH secretion (PubMed:22902404). Probable cell surface coreceptor for VEGFR2 involved in VEGFR2-mediated angiogenesis (PubMed:27834687). |
| Cellular Location | Secreted. Cell surface. Note=Secreted and tethered at the cell surface. |
| Tissue Location | Expressed in a broad spectrum of adult tissues (PubMed:12270931). |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
SCUBE2 Antibody: SCUBE2 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and are expressed in embryonic neuroectoderm and vascular epithelium. SCUBE2 is a close homolog to SCUBE1 and can form a heterodimeric complex with SCUBE1. SCUBE2 is thought to participate in the Hedgehog signaling pathway by specifically interacting with Sonic Hedgehog (SHH) and the receptor PTCH1, enhancing the SHH signaling activity in the raft microdomains of the plasma membrane, and may thus play important roles in organ development and tumor progression.
REFERENCES
Grimmond S, Larder R, Van Hateren N, et al. Cloning, mapping, and expression analysis of a gene encoding a novel mammalian EGF-related protein (SCUBE1). Genomics2000; 70:74-81.
Yang RB, Ng CK, Wasserman SM, et al. Identification of a novel family of cell-surface proteins expressed in human vascular endothelium. J. Biol. Chem.2002; 277:46364-73.
Grimmond S, Larder R, Van Hateren N, et al. Cloning, mapping, and expression analysis of a gene encoding a novel mammalian EGF-related protein (SCUBE1). Genomics2000; 70:74-81.
Tsai M, Cheng C, Lin Y, et al. Isolation and characterization of a secreted, cell-surface glycoprotein SCUBE2 from humans. Biochem. J. 2009; 422:119-28.
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