癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
ATP11B Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, ICC, E |
|---|---|
| Primary Accession | Q9Y2G3 |
| Other Accession | NP_055431, 62632750 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 134190 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | ATP11B antibody can be used for detection of ATP11B by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 23200 |
|---|---|
| Other Names | Probable phospholipid-transporting ATPase IF, 3.6.3.1, ATPase IR, ATPase class VI type 11B, P4-ATPase flippase complex alpha subunit ATP11B, ATP11B, ATPIF, ATPIR, KIAA0956 |
| Target/Specificity | ATP11B; |
| Reconstitution & Storage | ATP11B antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | ATP11B Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | ATP11B |
|---|---|
| Synonyms | ATPIF, ATPIR, KIAA0956 |
| Function | Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids, phosphatidylserines (PS) and phosphatidylethanolamines (PE), from the outer to the inner leaflet of intracellular membranes (PubMed:30018401). May contribute to the maintenance of membrane lipid asymmetry in endosome compartment (PubMed:30018401). |
| Cellular Location | Recycling endosome membrane; Multi-pass membrane protein. Early endosome. Endoplasmic reticulum. Golgi apparatus, trans-Golgi network. Note=Exit from the endoplasmic reticulum requires the presence of CDC50A, but not CDC50B (PubMed:21914794). In the presence of CDC50A, mainly located in recycling endosomes (PubMed:21914794). |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
ATP11B Antibody: ATP11B is a widely expressed integral membrane ATPase and is thought to drive the transport of phospholipids across membranes. It has been suggested that ATP11B is hormonally regulated and plays a role in the subnuclear trafficking of transcription factors with RING motifs. While the exact molecule ATP11B transports is unknown, increased mRNA expression of the homologous ATP11A has been observed in cells resistant to anti-cancer drugs such as farnesyltransferase inhibitors (FTIs), suggesting that ATP11B may also play a role in cell survival under harsh conditions.
REFERENCES
Mansharamani M, Hewetson A, and Chilton BS. Cloning and characterization of an atypical Type IV P-type ATPase that binds to the RING motif of RUSH transcription factors. J. Biol. Chem.2001; 276:3641-9.
Halleck MS, Lawler JF Jr, Blackshaw S, et al. Differential expression of putative transbilayer amphipath transporters. Physiol. Genomics1999; 1:139-50.
Zhang B, Groffen J, and Heisterkamp N. Resistance to farnesyltransferase inhibitors in Bcr/Abl-positive lymphoblastic leukemia by increased expression of a novel ABC transporter homolog ATP11a. Blood2005; 106:1355-61.
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