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LMBRD1 Antibody
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Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | Q9NUN5 |
| Other Accession | NP_060838, 261878497 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 61389 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | LMBRD1 antibody can be used for detection of LMBRD1 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 55788 |
|---|---|
| Other Names | Probable lysosomal cobalamin transporter, HDAg-L-interacting protein NESI, LMBR1 domain-containing protein 1, Nuclear export signal-interacting protein, LMBRD1, C6orf209, NESI |
| Target/Specificity | LMBRD1; |
| Reconstitution & Storage | LMBRD1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | LMBRD1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | LMBRD1 (HGNC:23038) |
|---|---|
| Synonyms | C6orf209, NESI |
| Function | Lysosomal membrane chaperone required to export cobalamin (vitamin B12) from the lysosome to the cytosol, allowing its conversion to cofactors (PubMed:19136951). Targets ABCD4 transporter from the endoplasmic reticulum to the lysosome (PubMed:27456980). Then forms a complex with lysosomal ABCD4 and cytoplasmic MMACHC to transport cobalamin across the lysosomal membrane (PubMed:25535791). Acts as an adapter protein which plays an important role in mediating and regulating the internalization of the insulin receptor (INSR) (By similarity). Involved in clathrin-mediated endocytosis of INSR via its interaction with adapter protein complex 2 (By similarity). Essential for the initiation of gastrulation and early formation of mesoderm structures during embryogenesis (By similarity). |
| Cellular Location | Endoplasmic reticulum membrane. Lysosome membrane; Multi-pass membrane protein. Cell membrane {ECO:0000250|UniProtKB:Q8K0B2}; Multi-pass membrane protein. Cytoplasmic vesicle, clathrin-coated vesicle {ECO:0000250|UniProtKB:Q8K0B2} |
| Tissue Location | Isoform 3 is expressed in liver. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
LMBRD1 Antibody: LMBRD1, also known as NESI (nuclear export signal-interacting protein, is a lysosomal membrane protein that is thought be involved in the transport and metabolism of cobalamin. LMBRD1 was initially identified as interacting with the large form of the hepatitis delta antigen and may be required for the nucleocytoplasmic shuttling of the hepatitis delta virus. Mutations in this gene are associated with the vitamin B12 metabolism disorder termed, homocystinuria-megaloblastic anemia complementation type F (cblF).
REFERENCES
Gailus S, Hohne W, Gasnier B, et al. Insights into lysosomal cobalamin trafficking: lessons learned from cblF disease. J. Mol. Med.2010; 88:459-66.
Wang Y-S, Chang SC, Huang C, et al. Novel nuclear export signal-interacting protein, NESI, critical for the assembly of hepatitis delta virus. J. Virol.2005; 79:8113-20.
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