癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
IRF4 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, ICC, E |
|---|---|
| Primary Accession | Q15306 |
| Other Accession | NP_002451, 167555104 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 51772 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | IRF4 antibody can be used for detection of IRF4 by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 3662 |
|---|---|
| Other Names | Interferon regulatory factor 4, IRF-4, Lymphocyte-specific interferon regulatory factor, LSIRF, Multiple myeloma oncogene 1, NF-EM5, IRF4, MUM1 |
| Target/Specificity | IRF4; IRF4 antibody is predicted to not cross-react with other IRF protein family members. IRF4 often migrates at a higher than predicted molecular weight in SDS-PAGE. |
| Reconstitution & Storage | IRF4 antibody can be stored at 4 ℃, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | IRF4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | IRF4 {ECO:0000303|PubMed:15489334, ECO:0000303|PubMed:8921401} |
|---|---|
| Function | Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA- 3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes. |
| Cellular Location | Nucleus. Cytoplasm |
| Tissue Location | Lymphoid cells. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
IRF4 Antibody: Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. Another member, IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF4 expression is tightly regulated in resting primary T cells and plays an essential role in the homeostasis and function of mature lymphocytes. IRF4 is induced by Toll-like receptor (TLR) activation and acts as a negative regulator of TLR signaling.
REFERENCES
Malmgaard L. Induction and regulation of IFNs during viral infections. J. Interferon & Cyto. Res. 2004; 24:439-54
Sato M, Suemori H, Hata N, et al. Distinct and essential roles of transcription factors IRF-3 and IRF-7 in response to viruses for IFN-alpha/beta gene induction. Immunity 2000; 13:539-48.
Mittrucker HW, Matsuyama T, Grossman A, et al. Requirement for the transcription factor LSIRF/IRF4 for mature B and T lymphocyte function. Science 1997; 275:540-3.
Negishi H, Ohba Y, Yanai H, et al. Negative regulation of Toll-like-receptor signaling by IRF4. Proc. Natl. Acad. Sci. USA 2005; 102:15989-94
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