癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
TYW2 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, ICC, E |
|---|---|
| Primary Accession | Q53H54 |
| Other Accession | Q53H54, 157388919 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 50236 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | TYW2 antibody can be used for detection of TYW2 by Western blot at 0.5 µg/mL. Antibody can also be used for immunocytochemistry starting at 10 µg/mL. |
| Gene ID | 55039 |
|---|---|
| Other Names | tRNA wybutosine-synthesizing protein 2 homolog, tRNA-yW-synthesizing protein 2, 2.5.1.114, tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase, TRMT12, TRM12, TYW2 |
| Target/Specificity | TRMT12; TYW2 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW2 are known to exist; this antibody will detect both isoforms |
| Reconstitution & Storage | TYW2 antibody can be stored at 4 ℃, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | TYW2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TRMT12 |
|---|---|
| Synonyms | TRM12, TYW2 |
| Function | S-adenosyl-L-methionine-dependent transferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S- adenosyl-L-methionine to the C-7 position of 4-demethylwyosine (imG-14) to produce wybutosine-86. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
TYW2 Antibody: TYW2, also known as TRM12, is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW2 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW2 was found to be amplified in 7 of 8 breast cancers cell lines analyzed, and qPCR analysis of 30 breast tumors showed overexpression of TYW2 mRNA of over 2-fold in 26, suggesting that TYW2 may play a role in breast cancer.
REFERENCES
Noma A and Suzuki T. Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis. Nucleic Acids Symp. Ser. (Oxf) 2006; 65-6
Noma A, Kirino Y, Ikeuchi Y, et al. Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA. EMBO J. 2006; 25:2142-54
Rodriquez V, Chen Y, Elkahloun A, et al. Chromosome 8 BAC array comparative genomic hybridization and expression analysis identify amplification and overexpression of TRMT12 in breast cancer. Genes Chromosomes Cancer 2007; 46:694-707
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