癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
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ATAD3A Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, ICC, E |
|---|---|
| Primary Accession | Q9NVI7 |
| Other Accession | Q9NVI7, 283436220 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 66218 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | ATAD3A antibody can be used for detection of ATAD3A by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 20 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 55210 |
|---|---|
| Other Names | ATPase family AAA domain-containing protein 3A, ATAD3A |
| Target/Specificity | ATAD3A; ATAD3A antibody is predicted to not cross-react with other ATAD protein family members. At least two isoforms are known to exist. |
| Reconstitution & Storage | ATAD3A antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | ATAD3A Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ATAD3A {ECO:0000303|PubMed:37832546, ECO:0000312|HGNC:HGNC:25567} |
|---|---|
| Function | Essential for mitochondrial network organization, mitochondrial metabolism and cell growth at organism and cellular level (PubMed:17210950, PubMed:20154147, PubMed:22453275, PubMed:31522117, PubMed:37832546, PubMed:39116259). May play an important role in mitochondrial protein synthesis (PubMed:22453275). May also participate in mitochondrial DNA replication (PubMed:17210950). May bind to mitochondrial DNA D-loops and contribute to nucleoid stability (PubMed:17210950). Required for enhanced channeling of cholesterol for hormone-dependent steroidogenesis (PubMed:22453275). Involved in mitochondrial-mediated antiviral innate immunity (PubMed:31522117). Required to protect mitochondria from the PERK-mediated unfolded protein response: specifically inhibits the activity of EIF2AK3/PERK at mitochondria-endoplasmic reticulum contact sites, thereby providing a safe haven for mitochondrial protein translation during endoplasmic reticulum stress (PubMed:39116259). Ability to inhibit EIF2AK3/PERK is independent of its ATPase activity (PubMed:39116259). Also involved in the mitochondrial DNA damage response by promoting signaling between damaged genomes and the mitochondrial membrane, leading to activation of the integrated stress response (ISR) (PubMed:37832546). |
| Cellular Location | Mitochondrion inner membrane; Single-pass membrane protein. Mitochondrion matrix, mitochondrion nucleoid Note=In the mitochondrial inner membrane, enriched in sites with the potential to form contacts with the outer membrane (PubMed:20154147, PubMed:20349121). The N-terminal domain interacts with the inner surface of the mitochondrial outer membrane and the C-terminal domain localizes in a specific matrix compartment, where it is associated with nucleoids (PubMed:18063578). Also present at mitochondria-endoplasmic reticulum contact sites; where it interacts with EIF2AK3/PERK (PubMed:39116259). |
| Tissue Location | Overexpressed in lung adenocarcinomas (at protein level). |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
ATAD3A Antibody: ATAD3A is a member of the AAA ATPase family, a family of proteins that catalyze ATP into ADP and are involved in several cellular functions such as cell-cycle regulation, protein proteolysis and transport. The AAA ATPase family is characterized by a highly conserved AAA motif containing Walker homology sequences and imparting ATPase activity. Mitochondrial membrane proteins ATAD3A/B contribute to the stabilization of nucleoids and may participate in the transformation pathway and the chemosensitivity of oligodendrogliomas. The gene encoding ATAD3A/B/C maps to human chromosome 1 and has been suggested to be an anti-apoptotic marker.
REFERENCES
Patel S and Latterich M. The AAA team: related ATPases with diverse functions. Trends Cell Biol. 1998; 8:65-71.
Ogura T and Wilkinson AJ. AAA+ superfamily ATPases: common structure—diverse function. Genes Cells 2001; 6:575-97.
Hubstenberger A, Labourdette G, Baudier J, et al. ATAD3A and ATAD3B are distal 1p-located genes differentially expressed in human glioma cell lines and present in vitro anti-oncogenic and chemoresistant properties. Exp. Cell Res. 2008; 314:2870-83.
Fang HY, Chang CL, Hsu SH, et al. ATPase family AAA domain-containing 3A is a novel anti-apoptotic factor in lung adenocarcinoma cells. J. Cell Sci. 2010; 123:1171-80.
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