癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
TMEM147 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | Q9BVK8 |
| Other Accession | NP_116024, 14249166 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 25261 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | TMEM147 antibody can be used for detection of TMEM147 by Western blot at 1 - 2 µg/mL. |
| Gene ID | 10430 |
|---|---|
| Other Names | Transmembrane protein 147, Protein NIFIE 14, TMEM147 |
| Target/Specificity | TMEM147; |
| Reconstitution & Storage | TMEM147 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | TMEM147 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | TMEM147 {ECO:0000303|PubMed:20538592, ECO:0000312|HGNC:HGNC:30414} |
|---|---|
| Function | Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes (PubMed:32820719, PubMed:36261522). The MPT complex takes over after the SEC61 complex: following membrane insertion of the first few transmembrane segments of proteins by the SEC61 complex, the MPT complex occludes the lateral gate of the SEC61 complex to promote insertion of subsequent transmembrane regions (PubMed:36261522). Also acts as a negative regulator of CHRM3 function, most likely by interfering with its trafficking to the cell membrane (PubMed:21056967). Negatively regulates CHRM3-mediated calcium mobilization and activation of RPS6KA1/p90RSK activity (PubMed:21056967). Regulates LBR localization to the nucleus inner membrane (PubMed:32694168). |
| Cellular Location | Endoplasmic reticulum membrane; Multi-pass membrane protein. Nucleus membrane; Multi- pass membrane protein. Cell membrane {ECO:0000250|UniProtKB:I6VSD2}; Multi-pass membrane protein |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
TMEM147 Antibody: TMEM147 is a recently identified protein that associates with Nicalin and NOMO, forming a complex that is thought to be similar to the gamma-secretase complex. TMEM147 localizes to the endoplasmic reticulum (ER), and in zebrafish, is expressed during early development. TMEM147 also interacts with the M3 muscarinic receptor (M3R) in the ER and is thought to negatively its expression and function.
REFERENCES
Dettmer U, Kuhn PH, Abou-Ajram C, et al. Transmembrane protein 147 (TMEM147) is a novel component of the Nicalin-NOMO protein complex. J. Biol. Chem. 2010; 285:26174-81
Rosemond E, Rossi M, McMillin SM, et al. Regulation of M3 muscarinic receptor expression and function by transmembrane protein 147. Mol. Pharmacol. 2011; 79:251-61.
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