癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
DDA Antibody
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Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | Q9BW61 |
| Other Accession | NP_003253, 13129016 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 11835 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | DDA1 antibody can be used for detection of DDA1 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 79016 |
|---|---|
| Other Names | DET1- and DDB1-associated protein 1, Placenta cross-immune reaction antigen 1, PCIA-1, DDA1, C19orf58, PCIA1 |
| Target/Specificity | DDA1; |
| Reconstitution & Storage | DDA antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | DDA Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DDA1 {ECO:0000303|PubMed:17452440, ECO:0000312|HGNC:HGNC:28360} |
|---|---|
| Function | Functions as a component of numerous distinct DCX (DDB1-CUL4- X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:17452440, PubMed:28302793, PubMed:28437394, PubMed:31686031, PubMed:31819272). In the DCX complexes, acts as a scaffolding subunit required to stabilize the complex (PubMed:31686031, PubMed:31819272). |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
DDA Antibody: DDA1 (DET1 and DDB1 associated 1), along with DET1 and DDB1 and a member of the UBE2E group of canonical ubiquitin-conjugating enzymes, comprise DDD-E2 complexes, which interact with multiple ubiquitin E3 ligases. One of these E3 ligases is Cul4-containing E3 ligase complex CRL4. Cells depleted of DDA1 spontaneously accumulate double-stranded DNA breaks in a similar fashion as Cul4A-, Cul4B-, or WDR23-depleted cells, suggesting that DDA1 interacts with the CRL4 complex and may be involved in the ubiquitination and subsequent proteasomal degradation of target proteins.
REFERENCES
Pick E, Lau O, Tsuge T, et al. Mammalian DET1 regulates Cul4A activity and forms stable complexes with E2 ubiquitin-conjugating enzymes. Mol. Cell. Biol. 2007; 27:4708-19.
Olma MH, Roy M, Le Bihan T, et al. An interaction network of the mammalian COP9 signalsome identifies Dda1 as a core subunit of multiple Cul4-based E3 ligases. J. Cell Sci. 2009; 122:1035-44
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