癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
ANKLE1 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | Q8NAG6 |
| Other Accession | NP_689576, 168229256 |
| Reactivity | Human, Mouse, Rat |
| Host | Chicken |
| Clonality | Polyclonal |
| Isotype | IgY |
| Calculated MW | 66890 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | ANKLE1 antibody can be used for detection of ANKLE1 by Western blot at 1 - 2 µg/mL. |
| Gene ID | 126549 |
|---|---|
| Other Names | Ankyrin repeat and LEM domain-containing protein 1, Ankyrin repeat domain-containing protein 41, LEM-domain containing protein 3, ANKLE1, ANKRD41, LEM3 |
| Target/Specificity | ANKLE1; Two isoforms of ANKLE1 are known to exist. ANKLE1 antibody is predicted to not cross-react with ANKLE2 |
| Reconstitution & Storage | ANKLE1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | ANKLE1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | ANKLE1 |
|---|---|
| Synonyms | ANKRD41, LEM3 |
| Function | Structure-selective DNA endonuclease that specifically cleaves branched DNA (PubMed:22399800, PubMed:27245214, PubMed:32866453, PubMed:36825683, PubMed:37216589). Processes chromatin bridges during cytokinesis by cleaving DNA, both priming TREX1-mediated resolution and directly resolving bridges (PubMed:36825683). Proper processing of chromatin bridges prevents their breakage during cytokinesis, thereby reducing DNA damage, micronuclei formation, and the activation of immune responses such as the cGAS-STING pathway (PubMed:36825683). Acts as a DNA tension sensor that resolves stretched chromatin bridges during cell division by cleaving mechanically stressed and supercoiled DNA (PubMed:41361016). Its activity increases in response to mechanical stress and negative supercoiling, specifically cleaving both strands of negatively supercoiled DNA at higher stretching forces (PubMed:41361016). |
| Cellular Location | Cytoplasm. Nucleus. Midbody. Note=At the steady state, localizes predominantly in the cytoplasm (PubMed:22399800, PubMed:27245214) Localizes to the midbody in a KIF23 and PRC1-dependent manner (PubMed:36825683). |
| Tissue Location | Expression is predominant in adult bone marrow. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
ANKLE1 Antibody: Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network. ANKLE1 (Ankyrin repeat and LEM domain containing 1) is a member of ankyrin repeat and LEM domain containing family. It is a potential single-pass membrane protein that contains ankyrin repeats and a Lem domain. Ankyrin repeats mediate protein-protein interactions and are among the most common structural motifs in known proteins. While the function of this protein is still under investigation, its gene locus has been associated with susceptibility to ovarian and breast cancer.
REFERENCES
Lee KK and Wilson KL. All in the family: evidence for four new LEM-domain proteins Lem2 (NET-25), Lem3, Lem4 and Lem5 in the human genome. Symp. Soc. Exp. Biol. 2004; 56:329-39.
Bolton KL, Tyrer J, Song H, et al. Common variants at 19p13 are associated with susceptibility to ovarian cancer. Nat. Genet. 2010; 42:880-4.
Antoniou AC, Wang X, Fredericksen ZS, et al. A locus on 19p13 modifies risk of breast cancer in BRCA1 mutation carriers and is associated with hormone receptor-negative breast cancer in the general population. Nat. Genet. 2010; 42:885-92.
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