癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
SIVA Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E |
|---|---|
| Primary Accession | O15304 |
| Other Accession | NP_068355, 11277468 |
| Reactivity | Human, Mouse |
| Host | Chicken |
| Clonality | Polyclonal |
| Isotype | IgY |
| Calculated MW | 18695 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | SIVA antibody can be used for detection of EPAC1 by Western blot at 1 µg/mL. Antibody can also be used for immunofluorescence starting at 20 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 10572 |
|---|---|
| Other Names | Apoptosis regulatory protein Siva, CD27-binding protein, CD27BP, SIVA1, SIVA |
| Target/Specificity | SIVA1; |
| Reconstitution & Storage | SIVA antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | SIVA Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | SIVA1 |
|---|---|
| Synonyms | SIVA |
| Function | Induces CD27-mediated apoptosis. Inhibits BCL2L1 isoform Bcl- x(L) anti-apoptotic activity. Inhibits activation of NF-kappa-B and promotes T-cell receptor-mediated apoptosis. |
| Cellular Location | Cytoplasm. Nucleus. Note=In the nucleus, accumulates in dot- like structures |
| Tissue Location | Ubiquitous. Mostly expressed in thymus, testis, ovary, prostate, small intestine and spleen and less in colon |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
SIVA Antibody: SIVA plays an important role in the apoptotic pathway induced by the CD27 antigen, a member of the tumor necrosis factor receptor (TFNR) superfamily. SIVA is a small cysteine-rich protein with a unique N-terminal amphipathic helical region, a death domain homology region (DDHR) and a C-terminal cysteine-rich B-box-like or zinc-finger-like structure. The DDHR domain binds to CD27 and induces apoptosis in T cells through a caspase-dependent mitochondrial pathway. SIVA inhibits BCL2L1 isoform Bcl-xL anti-apoptotic activity and inhibits activation of NFκ-B and promotes T-cell receptor-mediated apoptosis. SIVA represents a potential therapeutic target for the treatment of neuronal cell death diseases.
REFERENCES
Prasad KV, Ao Z, Yoon Y, et al. CD27, a member of the tumor necrosis factor receptor family, induces apoptosis and binds to Siva, a proapoptotic protein. Proc. Natl. Acad. Sci. USA 1997; 94:6346-51.
Py B, Slomianny C, Auberger P, et al. Siva-1 and an alternative splice form lacking the death domain, Siva-2, similarly induce apoptosis in T lymphocytes via a caspase-dependent mitochondrial pathway. J. Immunol. 2004; 172:4008-17.
Resch U, Schichl YM, Winsauer G, et al. Siva1 is a XIAP-interacting protein that balances NFkappaB and JNK signalling to promote apoptosis. J. Cell Sci. 2009; 122:2651-61
Jacobs SB, Basak S, Murray JI, et al. Siva is an apoptosis-selective p53 target gene important for neuronal cell death. Cell Death Differ. 2007; 14:1374-85.
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