癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
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NCLN Antibody
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Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | Q969V3 |
| Other Accession | NP_064555, 51873031 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 62974 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | NCLN antibody can be used for detection of NCLN by Western blot at 0.5 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 2.5 µg/mL. |
| Gene ID | 56926 |
|---|---|
| Other Names | Nicalin, Nicastrin-like protein, NCLN |
| Target/Specificity | NCLN; |
| Reconstitution & Storage | NCLN antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | NCLN Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NCLN {ECO:0000303|PubMed:36261522, ECO:0000312|HGNC:HGNC:26923} |
|---|---|
| Function | Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes (PubMed:32820719, PubMed:36261522). The MPT complex takes over after the SEC61 complex: following membrane insertion of the first few transmembrane segments of proteins by the SEC61 complex, the MPT complex occludes the lateral gate of the SEC61 complex to promote insertion of subsequent transmembrane regions (PubMed:36261522). May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning, via its interaction with NOMO (By similarity). |
| Cellular Location | Endoplasmic reticulum membrane; Single-pass membrane protein |
| Tissue Location | Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
NCLN Antibody: Nicalin, along with its binding partner Nomo, is a novel signaling antagonist to Nodals, signaling factors of the TGF-β superfamily that play a key role in vertebrate development. Nicalin is distantly related to Nicastrin, a component of the Alzheimer's disease-associated gamma-secretase, and is thought to have a similar function. Another protein, TMEM147, has also been recently identified as associating with Nicalin and NOMO as part of the gamma-secretase-like complex.
REFERENCES
Haffner C, Frauli M, Topp S, et al. Nicalin and its binding partner Nomo are novel Nodal signaling antagonists. EMBO J. 2004; 23:3041-50.
Dettmer U, Kuhn PH, Abou-Ajram C, et al. Transmembrane protein 147 (TMEM147) is a novel component of the Nicalin-NOMO protein complex. J. Biol. Chem. 2010; 285:26174-81.
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