癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
ZF21 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E |
|---|---|
| Primary Accession | Q9BQ24 |
| Other Accession | NP_001185882, 312147341 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 26506 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | ZF21 antibody can be used for detection of ZF21 by Western blot at 1 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 79038 |
|---|---|
| Other Names | Zinc finger FYVE domain-containing protein 21, ZF21, ZFYVE21 |
| Target/Specificity | ZFYVE21; At least two isoforms of ZF21 are known to exist; this antibody will detect both isoforms. |
| Reconstitution & Storage | ZF21 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | ZF21 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | ZFYVE21 |
|---|---|
| Function | Plays a role in cell adhesion, and thereby in cell motility which requires repeated formation and disassembly of focal adhesions. Regulates microtubule-induced PTK2/FAK1 dephosphorylation, an event important for focal adhesion disassembly, as well as integrin beta- 1/ITGB1 cell surface expression. |
| Cellular Location | Cell junction, focal adhesion. Cytoplasmic vesicle. Endosome. Note=Within cytoplasmic vesicles, partially colocalizes with EEA1, an endosomal marker |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
ZF21 Antibody: ZF21 was initially identified as protein that could bind to the cytoplasmic tail of MT1-MMP (Membrane-type 1 matrix metalloproteinase), a potent invasion-promoting protease. ZF21 is a member of a protein family characterized by the presence of a phosphatidylinositol 3-phosphate-binding FYVE domain and regulates focal adhesions (FAs) and cell movement. Knockdown of ZF21 expression resulted in a delay of FA disassembly following induction of synchronous disassembly of FAs by nocodazole treatment, suggesting that ZF21 is involved in FA disassembly. ZF21 contains a noncanonical pleckstrin homology domain that is a possible therapeutic target to treat metastatic cancer.
REFERENCES
Uekita T, Gotoh I, Kinoshita T, et al. Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein-1 is a new member of the Cupin superfamily. A possible multifunctional protein acting as an invasion suppressor down-regulated in tumors. J. Biol. Chem. 2004; 279:12734-43.
Nagano M, Hoshino D, Sakamoto T, et al. ZF21 protein regulates cell adhesion and motility. J. Biol. Chem. 2010; 285:21013-22.
Nagano M, Hoshino D, Koshiba S, et al. ZF21 protein, a regulator of the disassembly of focal adhesions and cancer metastasis, contains a novel noncanonical pleckstrin homology domain. J. Biol. Chem. 2011; 286:31598-609.
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