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POU3F2 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E |
|---|---|
| Primary Accession | P20265 |
| Other Accession | NP_005595, 51702521 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 46893 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | POU3F2 antibody can be used for detection of POU3F2 by Western blot at 1 - 2 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 5454 |
|---|---|
| Other Names | POU domain, class 3, transcription factor 2, Brain-specific homeobox/POU domain protein 2, Brain-2, Brn-2, Nervous system-specific octamer-binding transcription factor N-Oct-3, Octamer-binding protein 7, Oct-7, Octamer-binding transcription factor 7, OTF-7, POU3F2, BRN2, OCT7, OTF7 |
| Target/Specificity | POU3F2; At least two isoforms are known to exist. This antibody will recognize both isoforms. POU3F2 antibody is predicted to not cross-react with other members of the POU domain class 3 family. |
| Reconstitution & Storage | POU3F2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | POU3F2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | POU3F2 |
|---|---|
| Synonyms | BRN2, OCT7, OTF7 |
| Function | Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (By similarity). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity). |
| Cellular Location | Nucleus. |
| Tissue Location | Expressed specifically in the neuroectodermal cell lineage |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
POU3F2 Antibody: POU3F2, also commonly known as brain-2, is a member of a family of POU domain genes expressed in mouse brain and is thought to be involved in the development of the neocortex and establishment of neural cell lineage. Recent studies suggest that POU3F2 may be involved with the development of neurodegenerative diseases as well as tumor development and proliferation. Along with the neural-lineage-specific transcription factors ASCL1 and MYT1L, POU3F2 can convert fibroblasts to functional neurons in vitro, a form of artificial stem cells termed induced neuronal (iN) cells, suggesting that these cells may be useful in the treatment of neurodegenerative diseases.
REFERENCES
Hara Y, Rovescalli AC, Kim Y, et al. Structure and evolution of four POU domain genes expressed in mouse brain. Proc. Natl. Acad. Sci. USA 1992; 89:3280-4.
McEvilly RJ, de Diaz MO, Schonemann MD, et al. Transcriptional regulation of cortical neuron migration by POU domain factors. Science 2002; 295:1528-32.
Huang YT, Iwamoto K, Kurosaki T, et al. The neuronal POU transcription factor Brn-2 interacts with Jab1, a gene involved in the onset of neurodegenerative diseases. Neurosci. Lett. 2005; 382:175-8
Goodall J, Carreira S, Denat L, et al. Brn-2 represses microphthalmia-associated transcription factor expression and marks a distinct subpopulation of microphthalmia-associated transcription factor-negative melanoma cells. Cancer Res. 2008; 68:7788-94
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