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MECR Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | Q9BV79 |
| Other Accession | NP_057095, 67078404 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 40462 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | MECR antibody can be used for detection of MECR by Western blot at 1 - 2 µg/mL. |
| Gene ID | 51102 |
|---|---|
| Other Names | Trans-2-enoyl-CoA reductase, mitochondrial, 1.3.1.38, Nuclear receptor-binding factor 1, HsNrbf-1, NRBF-1, MECR, NBRF1 |
| Target/Specificity | MECR; At least two isoforms of MECR are known to exist; this antibody will detect both isoforms. |
| Reconstitution & Storage | MECR antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | MECR Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MECR |
|---|---|
| Synonyms | NBRF1 |
| Function | Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro. Displays a preference for medium-chain over short- and long-chain substrates (PubMed:12654921, PubMed:18479707, PubMed:27817865). May provide the octanoyl chain used for lipoic acid biosynthesis, regulating protein lipoylation and mitochondrial respiratory activity particularly in Purkinje cells (By similarity). Involved in iron homeostasis; affecting Fe-S cluster assembly and ceramide metabolism (PubMed:37653044). Required for proper morphology and bioenergetic functions of mitochondria (PubMed:37653044). Required for maintenance of neurons (By similarity). |
| Cellular Location | [Isoform 1]: Mitochondrion |
| Tissue Location | Highly expressed in skeletal and heart muscle. Expressed at lower level in placenta, liver, kidney and pancreas Weakly or not expressed in lung. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
MECR Antibody: The mitochondrial trans-2-enoyl-CoA reductase (MECR), was initially identified as nuclear receptor-binding factor 1 (NRBF1), which can interact with a multitude of nuclear hormone receptors in the presence of the respective ligands. MECR has been shown to be part of the mitochondrial fatty acid synthesis (FAS II) system and to catalyze the NAPDH-dependent reduction of 2-enoyl thioesters, generating saturated acyl-groups. Overexpression of this gene in transgenic mice can lead to cardiac abnormalities, suggesting that inappropriate expression of genes of FAS II can result in the development of hereditary cardiomyopathy.
REFERENCES
Masuda N, Yasumo H, Furusawa T, et al. Nuclear receptor binding factor-1 (NRBF-1), a protein interacting with a wide spectrum of nuclear hormone receptors. Gene 1998; 221:225-33.
Chen Z, Leskinen H, Liimatta E, et al. Myocardial overexpression of Mecr, a gene of mitochondrial FAS II leads to cardiac dysfunction in mouse. PLoS One 2009; 4:e5589.
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