ESR1 Antibody
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- 实验流程
- 背景知识
Application ![]()
| WB, IF, E, IHC-P |
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Primary Accession | P03372 |
Other Accession | NP_001116214, 170295804 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 66216 Da |
Concentration (mg/ml) | 1 mg/mL |
Conjugate | Unconjugated |
Application Notes | ESR1 antibody can be used for detection of ESR1 by Western blot at 1 - 2 µg/mL. |
Gene ID | 2099 |
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Other Names | Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1, ESR1, ESR, NR3A1 |
Target/Specificity | ESR1; ESR1 antibody is human, mouse and rat reactive. |
Reconstitution & Storage | ESR1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
Precautions | ESR1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ESR1 |
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Synonyms | ESR, NR3A1 |
Function | Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE- independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa- B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (PubMed:17922032). Maintains neuronal survival in response to ischemic reperfusion injury when in the presence of circulating estradiol (17-beta-estradiol/E2) (By similarity). |
Cellular Location | [Isoform 1]: Nucleus {ECO:0000255|PROSITE- ProRule:PRU00407, ECO:0000269|PubMed:12682286, ECO:0000269|PubMed:20074560}. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=A minor fraction is associated with the inner membrane Nucleus. Golgi apparatus. Cell membrane. Note=Colocalizes with ZDHHC7 and ZDHHC21 in the Golgi apparatus where most probably palmitoylation occurs. Associated with the plasma membrane when palmitoylated |
Tissue Location | Widely expressed (PubMed:10970861). Not expressed in the pituitary gland (PubMed:10970861) |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
ESR1 Antibody: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors (1). Estrogen receptors, including ESR1, also known as ER-alpha and ESR2 (ER-beta), contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ESR1 is a widely expressed nuclear protein and serves as a strong activator of estrogen responsive genes (1,2). Phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), plays a large role in regulating ER alpha activity (3).
REFERENCES
Pakdel F, Reese JC, and Katzenellenbogen BS. Identification of charged residues in an N- terminal portion of the hormone-binding domain of the human estrogen receptor important in transcriptional activity of the receptor. Mol. Endocrinol. 1993; 7:1408-17.
Sheeler CQ, Singleton DW, and Khan SA. Mutation of serines 104, 106, and 118 inhibits dimerization of the human estrogen receptor in yeast. Endocr. Res. 2003; 29:237-55.
Rogatsky I, Trowbridge JM, and Garabedian MJ. Potentiation of human estrogen receptor alpha transcriptional activation through phosphorylation of serines 104 and 106 by the cyclin A-CDK2 complex. J. Biol. Chem. 1999; 274:22296-302.

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