癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
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FHIT Antibody
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- 实验流程
- 背景知识
Application 
| WB, IF, E |
|---|---|
| Primary Accession | P49789 |
| Other Accession | NP_002003, 4503719 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 16858 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | FHIT antibody can be used for detection of FHIT by Western blot at 1 - 2 µg/ml. |
| Gene ID | 2272 |
|---|---|
| Other Names | Bis(5'-adenosyl)-triphosphatase, 3.6.1.29, AP3A hydrolase, AP3Aase, Diadenosine 5', 5'''-P1, P3-triphosphate hydrolase, Dinucleosidetriphosphatase, Fragile histidine triad protein, FHIT |
| Target/Specificity | FHIT; FHIT antibody is human and mouse reactive. |
| Reconstitution & Storage | FHIT antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | FHIT Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | FHIT |
|---|---|
| Function | Possesses dinucleoside triphosphate hydrolase activity (PubMed:12574506, PubMed:15182206, PubMed:8794732, PubMed:9323207, PubMed:9543008, PubMed:9576908). Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP (PubMed:12574506, PubMed:15182206, PubMed:8794732, PubMed:9323207, PubMed:9543008, PubMed:9576908). Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP (PubMed:8794732). Exhibits adenylylsulfatase activity, hydrolyzing adenosine 5'-phosphosulfate to yield AMP and sulfate (PubMed:18694747). Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2 (PubMed:18694747). Exhibits adenylylsulfate-ammonia adenylyltransferase, catalyzing the ammonolysis of adenosine 5'- phosphosulfate resulting in the formation of adenosine 5'- phosphoramidate (PubMed:26181368). Also catalyzes the ammonolysis of adenosine 5-phosphorofluoridate and diadenosine triphosphate (PubMed:26181368). Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5 (PubMed:18077326). Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways (PubMed:16407838). Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis (PubMed:15313915). Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low-affinity Ca(2+) transporters, enhancing mitochondrial calcium uptake (PubMed:12574506, PubMed:19622739). Functions as a tumor suppressor (By similarity). |
| Cellular Location | Cytoplasm. Mitochondrion. Nucleus |
| Tissue Location | Low levels expressed in all tissues tested. Phospho-FHIT observed in liver and kidney, but not in brain and lung Phospho-FHIT undetected in all tested human tumor cell lines |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
FHIT is member of the histidine triad gene family and is a diadenosine involved in purine metabolism (1). FHIT is also thought to be a tumor suppressor gene and is involved in multiple apoptotic pathways (1,2). The FHIT gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene (3). Aberrant transcripts from this gene have been found in multiple carcinomas (4).
REFERENCES
Barnes LD, Garrison PN, Siprashvili Z, et al. Fhit, a putative tumor suppressor in humans, is a dinucleotide 5',5'''-P1,P3-triphosphate hydrolase. Biochemistry 1996; 35:11529-35.
Wali A. FHIT: doubts are clear now. ScientificWorldJournal 2010; 10:1142-51.
Ohta M, Inoue H, Cotticelli MG, et al. The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers. Cell 1996; 84:587-97.
Drusco A, Pekarsky Y, Costinean S, et al. Common fragile site tumor suppressor genes and corresponding mouse models of cancer. J. Biomed. Biotechnol.2011; Epub 2010 Dec 29.
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