癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
PHF14 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E |
|---|---|
| Primary Accession | O94880 |
| Other Accession | NP_055475, 55769548 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 106986 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | PHF14 antibody can be used for detection of PHF14 by Western blot at 1 - 2 µg/ml. |
| Gene ID | 9678 |
|---|---|
| Other Names | PHD finger protein 14, PHF14, KIAA0783 |
| Target/Specificity | PHF14; PHF14 antibody is human, mouse and rat reactive. Multiple isoforms of PHF14 are known to exist. |
| Reconstitution & Storage | PHF14 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | PHF14 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | PHF14 |
|---|---|
| Synonyms | KIAA0783 |
| Function | Histone-binding protein (PubMed:23688586). Binds preferentially to unmodified histone H3 but can also bind to a lesser extent to histone H3 trimethylated at 'Lys-9' (H3K9me3) as well as to histone H3 monomethylated at 'Lys-27' (H3K27ac) and trimethylated at 'Lys-27' (H3K27me3) (By similarity). Represses PDGFRA expression, thus playing a role in regulation of mesenchymal cell proliferation (By similarity). Suppresses the expression of CDKN1A/p21 by reducing the level of trimethylation of histone H3 'Lys-4', leading to enhanced proliferation of germinal center B cells (By similarity). |
| Cellular Location | [Isoform 1]: Nucleus. Chromosome. Note=Mainly localized in the nucleus of interphase cells. In mitotic cells, colocalizes with condensed chromatin during metaphase and anaphase. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
PHF14, a novel plant homology domain (PHD) transcription factor, controls the proliferation of mesenchymal cells by regulating PDGFR alpha expression in embryonic development (1). PHD finger transcription factors are concerned with transcriptional regulation by interacting with modified histones (2), and dysregulation of several genes encoding PHD finger transcription factors, such as ING1 and RBP2, has been found in human cancer diseases suggesting these factors have an important role for cancer cell proliferation. PHF14 is a potential target for new treatments of lung fibrosis (1, 3).
REFERENCES
Kitagawa M, Takebe A, Ono Y, et al. Phf14, a novel regulator of mesenchyme growth via platelet-derived growth factor (PDGF) receptor-a. J. Biol. Chem. 2012; 287:27983-96.
Musco G and Peterson P. PHD finger of autoimmune regulator: an epigenetic link between the histone modifications and tissue-specific antigen expression in thymus. Epigenetics 2008; 3:310-4.
Musselman CA and Kutateladze TG. PHD fingers: epigenetic effectors and potential drug targets. Mol. Interv. 2009; 9:314-23.
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