癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
ULK4 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | Q96C45 |
| Other Accession | NP_060356, 151301204 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 142442 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | ULK4 antibody can be used for detection of ULK4 by Western blot at 0.5 - 1 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 54986 |
|---|---|
| Other Names | Serine/threonine-protein kinase ULK4, 2.7.11.1, Unc-51-like kinase 4, ULK4 |
| Target/Specificity | ULK4; ULK4 antibody is human and mouse reactive. Multiple isoforms of ULK4 are known to exist. |
| Reconstitution & Storage | ULK4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | ULK4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | ULK4 {ECO:0000303|PubMed:32814032, ECO:0000312|HGNC:HGNC:15784} |
|---|---|
| Function | Pseudokinase that acts as a molecular adapter in the smoothened signaling pathway (PubMed:38096226). Required to relieve inhibition of the GLI2 transcription factor in the presence of hedgehog (DHH, IHH or SHH) ligand: acts by mediating the association between STK36 and its ligand GLI2 in ciliary tip, promoting GLI2 phosphorylation and dissociation from SUFU inhibitor, followed by GLI2 translocation to the nucleus (PubMed:38096226). |
| Cellular Location | Cell projection, cilium. Note=STK36 and ULK4 are mutually dependent for localization to ciliary tip |
| Tissue Location | Expressed in the brain, mainly in postmitotic neurons, including GABAergic neurons, but not in astrocytes (at protein level). |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
ULK4 belongs to the Ser/Thr protein kinase superfamily and plays a role in the ATP-dependent phosphorylation of target proteins (1). Knockout of ULK genes results in a severe defect in the autophagy pathway (2). ULK4, like the other Unc-51-like kinases such as ULK1, ULK2 and ULK3, is highly conserved among eukaryotes (3). ULK4 has been implicated as a gene involved in the development of hydrocephalus (3). This neurologic disorder is being used in animal models to elucidate factors responsible for the excessive accumulation of cerebrospinal fluid in hydrocephalic humans (4).
REFERENCES
Suzuki K, Kubota Y, Sekito T, et al. Hierarchy of Atg proteins in pre-autophagosomal structure organization. Genes to Cells 2007; 12:209–18.
Lee EJ and Tournier C. The requirement of uncoordinated 51-like kinase 1 (ULK1) and ULK2 in the regulation of autophagy. Autophagy 2011; 7:689-95.
Zhou X, Babu JR, da Silva S, et al. Unc-51-like kinase 1/2-mediated endocytic processes regulate filopodia extension and branching of sensory axons. Proc. Natl. Acad. Sci. USA 2007; 104:5842-7.
Vogel P, Read RW, Hansen GM, et al. Congenital hydrocephalus in genetically engineered mice. Vet. Pathol. 2012; 49:166-81.
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