癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
LAMTOR3 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | Q9UHA4 |
| Other Accession | NP_068805, 11496277 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 13623 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | LAMTOR3 antibody can be used for detection of LAMTOR3 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 8649 |
|---|---|
| Other Names | Ragulator complex protein LAMTOR3, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3, MEK-binding partner 1, Mp1, Mitogen-activated protein kinase kinase 1-interacting protein 1, Mitogen-activated protein kinase scaffold protein 1, LAMTOR3, MAP2K1IP1, MAPKSP1 |
| Target/Specificity | LAMTOR3; LAMTOR3 antibody is human, mouse and rat reactive. At least two isoforms of LAMTOR3 are known to exist; this antibody will detect both isoforms. LAMTOR3 antibody is predicted to not cross-react with other LAMTOR family proteins. |
| Reconstitution & Storage | LAMTOR3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | LAMTOR3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | LAMTOR3 (HGNC:15606) |
|---|---|
| Synonyms | MAP2K1IP1, MAPKSP1 |
| Function | As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids (PubMed:20381137, PubMed:22980980, PubMed:28935770, PubMed:29107538, PubMed:29123114, PubMed:29158492, PubMed:30181260). Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator plays a dual role for the small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD): it (1) acts as a guanine nucleotide exchange factor (GEF), activating the small GTPases Rag and (2) mediates recruitment of Rag GTPases to the lysosome membrane (PubMed:22980980, PubMed:28935770, PubMed:29107538, PubMed:29123114, PubMed:29158492, PubMed:30181260). Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated (PubMed:22980980, PubMed:28935770, PubMed:29107538, PubMed:29123114, PubMed:29158492, PubMed:30181260). Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2 (By similarity). |
| Cellular Location | Late endosome membrane {ECO:0000250|UniProtKB:O88653}; Peripheral membrane protein {ECO:0000250|UniProtKB:O88653}; Cytoplasmic side {ECO:0000250|UniProtKB:O88653}. Note=Recruited to lysosome and endosome membranes by LAMTOR1. {ECO:0000250|UniProtKB:O88653} |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The late endosomal/lysosomal adaptor MAPK and MTOR activator 3 (LAMTOR3) protein belongs to the LAMTOR family of proteins, and together with LAMTOR2 and the MAPK1 and ERK kinase 1 (MEK1) localizes to late endosomes where it is required for the efficient activation of ERK signaling (1,2). This complex is involved in the regulation of late endosomal traffic and cellular proliferation (3) and plays a role in cellular host defense against Salmonella infection (4).
REFERENCES
Wunderlich W, Fialka I, Teis D, et al. A novel 14-kilodalton protein interacts with the mitogen-activated protein kinase scaffold MP1 on a late endosomal/lysosomal compartment. J. Cell Biol. 2001; 152:765-76.
Teis D, Wunderlich W, and Huber LA. Localization of the MP1-MAPK scaffold complex to endosomes is mediated by p14 and required for signal transduction. Dev. Cell 2002; 3:803-14.
Teis D, Taub N, Kurzbauer R, et al. p14-MP1-MEK1 signaling regulates endosomal traffic and cellular proliferation during tissue homeostasis. J. Cell Biol. 2006; 175:861-8.
Taub N, Nairz M, Hilber D, et al. The late endosomal adaptor p14 is a macrophage host-defense factor against Salmonella infection. J. Cell Sci. 2012; 125:2698-708.
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