癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
TAF9 Antibody
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- 背景知识
Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | Q16594 |
| Other Accession | NP_003178, 4507351 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 28974 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | TAF9 antibody can be used for detection of TAF9 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL. |
| Gene ID | 6880 |
|---|---|
| Other Names | Transcription initiation factor TFIID subunit 9, RNA polymerase II TBP-associated factor subunit G, STAF31/32, Transcription initiation factor TFIID 31 kDa subunit, TAFII-31, TAFII31, Transcription initiation factor TFIID 32 kDa subunit, TAFII-32, TAFII32, TAF9, TAF2G, TAFII31 |
| Target/Specificity | TAF9; TAF9 antibody is human, mouse and rat reactive. Multiple isoforms of TAF9 are known to exist. TAF9 is predicted to not cross-react with other TAF family members. |
| Reconstitution & Storage | TAF9 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | TAF9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TAF9 |
|---|---|
| Synonyms | TAF2G, TAFII31 |
| Function | The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed:33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:33795473). TAF9 is also a component of the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex (PubMed:15899866). TAF9 and its paralog TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes (PubMed:15899866). Essential for cell viability (PubMed:15899866). May have a role in gene regulation associated with apoptosis (PubMed:15899866). |
| Cellular Location | Nucleus |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Initiation of transcription by RNA polymerase II is coordinated by the transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and the TBP-associated factors (TAFs). TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation (1). TAF9 is one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16 (2).
REFERENCES
Zawel L and Reinberg D. Advances in RNA polymerase II transcription. Curr. Opin. Cell Biol. 1992; 4:488-95.
Klemm RD, Goodrich JA, Zhou S, et al. Molecular cloning and expression of the 32-kDa subunit of human TFIID reveals interactions with VP16 and TFIIB that mediate transcriptional activation. Proc. Natl. Acad. Sci. USA 1995; 92:5788-92.
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