癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
DENR Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | O43583 |
| Other Accession | NP_003668, 27501446 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 22092 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | DENR antibody can be used for detection of DENR by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL. |
| Gene ID | 8562 |
|---|---|
| Other Names | Density-regulated protein, DRP, Protein DRP1, Smooth muscle cell-associated protein 3, SMAP-3, DENR, DRP1 |
| Target/Specificity | DENR; DENR antibody is human, mouse and rat reactive. |
| Reconstitution & Storage | DENR antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | DENR Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DENR |
|---|---|
| Synonyms | DRP1 |
| Function | Translation regulator forming a complex with MCTS1 to promote translation reinitiation. Translation reinitiation is the process where the small ribosomal subunit remains attached to the mRNA following termination of translation of a regulatory upstream ORF (uORF), and resume scanning on the same mRNA molecule to initiate translation of a downstream ORF, usually the main ORF (mORF). The MCTS1/DENR complex is pivotal to two linked mechanisms essential for translation reinitiation. Firstly, the dissociation of deacylated tRNAs from post- termination 40S ribosomal complexes during ribosome recycling. Secondly, the recruitment in an EIF2-independent manner of aminoacylated initiator tRNA to P site of 40S ribosomes for a new round of translation. This regulatory mechanism governs the translation of more than 150 genes which translation reinitiation is MCTS1/DENR complex-dependent. |
| Cellular Location | Cytoplasm. |
| Tissue Location | Highly expressed in heart and skeletal muscle and moderately expressed in the brain, placenta, liver and pancreas. Weakly expressed in the lung and kidney. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
DENR (density-regulated protein), also designated DRP or smooth muscle cell associated protein 3 (SMAP-3), is a 198 amino acid protein that may be involved in the translation of target mRNAs by scanning and recognition of the initiation codon (1). It plays a role in the modulation of the translational profile of a subset of cancer-related mRNAs when recruited to the translational initiation complex by the oncogene MCTS1 (2,3). DENR contains a SUI1 domain and is found in a variety of tissues with highest levels present in skeletal and cardiac muscle. It is up-regulated in ovarian and breast cancer cells by ERBB2 overexpression (4).
REFERENCES
Deyo JE, Chiao PJ, Tainsky MA. DRP, a novel protein expressed at high cell density but not during growth arrest. DNA Cell Biol. 1998; 17:437-47.
Skabkin MA, Skabkina OV, Dhote V, et al. Activities of Ligatin and MCT-1/DENR in eukaryotic translation initiation and ribosomal recycling. Genes Dev. 2010; 24:1787-801.
Mazan-Mamczarz K and Gartenhaus RB. Post-transcriptional control of the MCT1-associated protein DENR/DRP by RNA-binding protein AUF1. Cancer Genomics Proteomics 2007; 4:233-9.
Oh JJ, Grosshans DR, Wong SG et al. Identification of differentially expressed genes associated with HER-2/neu overexpression in human breast cancer cells. Nucleic Acids Res. 1999; 27:4008-17.
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