癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
ATMIN Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, ICC, E |
|---|---|
| Primary Accession | O43313 |
| Other Accession | NP_056066, 54792092 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 88348 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | ATMIN antibody can be used for detection of ATMIN by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunocytochemistry at 5 µg/mL. For Immunoflorescence start at 2.5 µg/mL. |
| Gene ID | 23300 |
|---|---|
| Other Names | ATM interactor, ATM/ATR-substrate CHK2-interacting zinc finger protein, ASCIZ, Zinc finger protein 822, ATMIN, KIAA0431, ZNF822 |
| Target/Specificity | ATMIN; ATMIN antibody is human, mouse and rat reactive. At least three isoforms of ATMIN are known to exist. |
| Reconstitution & Storage | ATMIN antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | ATMIN Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | ATMIN |
|---|---|
| Synonyms | KIAA0431, ZNF822 |
| Function | Transcription factor. Plays a crucial role in cell survival and RAD51 foci formation in response to methylating DNA damage. Involved in regulating the activity of ATM in the absence of DNA damage. May play a role in stabilizing ATM. Binds to the DYNLL1 promoter and activates its transcription. |
| Cellular Location | Nucleus. Note=Nuclear, in discrete foci during G1 phase |
| Tissue Location | Ubiquitously expressed in normal tissues and cancer cell lines with highest levels in placenta and skeletal muscle |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The ATM/ATR-substrate CHK2-interacting zinc finger protein (ATMIN), also known as ASCIZ, forms DNA damage-induced nuclear foci that contain the DNA repair protein Rad51 (1). ATMIN is also thought to be involved in embryonic development, as an absence of ATMIN causes late-embryonic lethality in mice with a range of organ development defects (2). It also activates the transcription DYNLL1, a light chain of the dynein motor complex and sequence-specific regulator of protein dimerization of numerous targets. DYNLL1 can bind to and inhibit the transcription activation domain of ATMIN, forming a simple dynamic feedback loop for DYNLL1 expression (3).
REFERENCES
McNees CJ, Conlan LA, Tenis N, et al. ASCIZ regulates lesion-specific Rad51 focus formation and apoptosis after methylating DNA damage. EMBO J. 2005; 24:2447-57.
Jurado S, Smyth I, van Denderen B, et al. Dual functions of ASCIZ in the DNA base damage response and pulmonary organogenesis. PLoS Genet. 2010; 6:e1001170.
Jurado S, Conlan LA, Baker EK, et al. ATM substrate Chk2-interating Zn2+ finger (ASCIZ) is a bi-functional transcriptional activator and feedback sensor in the regulation of Dynein Light Chain (DYNLL1) expression. J. Biol. Chem. 2012; 287:3156-64.
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